Mitochondria-Enhanced Preservation Solution for Cell Viability
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Solution Overview
Problem
Current preservation solutions are insufficient in maintaining the biological function of isolated tissues or organs for an extended period, leading to functional deterioration and increased discard rates in transplantation, as they fail to effectively preserve the activity of isolated cells, tissues, or organs during transportation.
Innovation Solution
A preservation solution composition comprising isolated mitochondria, along with histidine, tryptophan, and ketoglutarate, is developed to maintain the activity of isolated cells, tissues, or organs, which can be stored in a cold or cryopreservation solution to extend their viability for transplantation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional preservation solutions are used, then the isolated tissue or organ can be transported, but the biological function deteriorates over time
Solution Approach 1:
The patent changes the chemical composition parameters of the preservation solution by adding specific concentrations of histidine (5-50 mM), tryptophan (1-20 mM), and ketoglutarate (1-10 mM) to enhance mitochondrial function and maintain cellular energy production during extended preservation periods
Solution Approach 2:
The preservation solution combines multiple protective agents (histidine, tryptophan, ketoglutarate) with traditional preservation components to create a composite formulation that addresses multiple degradation mechanisms simultaneously, including oxidative stress, energy depletion, and mitochondrial dysfunction
2Reliability
If the isolated tissue or organ is transported quickly, then transplantation success rate increases, but delivery time is insufficient
Solution Approach 1:
The preservation solution is applied before transplantation to pre-condition the isolated tissue or organ, enhancing its resistance to ischemic injury and extending the viable window for transport and surgical preparation
Solution Approach 2:
The solution maintains continuous mitochondrial function and energy production throughout the preservation period, ensuring that cellular metabolism remains active and functional capabilities are preserved from isolation through transplantation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The preservation solution effectively maintains the activity and viability of isolated cells, tissues, or organs for a longer period, enhancing the success rate of transplantation by maintaining cellular energy production and metabolic functions, thus improving the efficiency of organ preservation and transportation.
Implementation Method 1
mitochondria, which are essential for producing adenosine triphosphate (ATP), an energy source
Data Source
AI summary
The present invention relates to a preservation solution composition for the preservation of an isolated cell, tissue, or organ, comprising isolated mitochondria; and a method for preserving an isolated cell, tissue, or organ by using the same. The preservation solution composition according to the present invention has excellent long-term preservability while maintaining the activity of isolated cell, tissue, or organ. Therefore, the present invention may be effectively used in the transplantation medicine field for ultimately improving transplant success rates and in the regenerative medicine field and the like.