Mixed Bacteria for Robinia pseudoacacia Nodulation
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Solution Overview
Problem
There is a lack of suitable legume vegetation nodule-promoting strains for different vegetations in China, particularly for Robinia pseudoacacia, which hinders the effective application of external-soil spray seeding greening technology.
Innovation Solution
A mixed bacteria composition including Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28 is provided, which is specifically designed to promote nodulation and nitrogen fixation in Robinia pseudoacacia, and is applied by diluting the fermentation broths and watering them on the rhizosphere soil of the seedlings.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If general soil bacteria are used in spray seeding substrate, then basic growth promotion function is provided, but specific nodule-promoting effect on Robinia pseudoacacia is insufficient
Solution Approach 1:
The patent segments the bacterial community into three specific functional strains (Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28) that are selected and isolated from Robinia pseudoacacia rhizosphere soil. Each strain performs specific functions: Kocuria sp. X-22 promotes nodule formation, Microbacterium sp. X-26 enhances nitrogen fixation, and Bacillus sp. X-28 improves plant growth. This segmentation allows targeted promotion of nodulation and nitrogen fixation specific to Robinia pseudoacacia.
Solution Approach 2:
The patent creates a composite bacterial formulation by combining three different bacterial strains (Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28) into a mixed bacteria preparation. This composite approach synergistically combines the nodule-promoting, nitrogen-fixing, and growth-enhancing capabilities of each strain, providing comprehensive and reliable nodule-promoting effects specific to Robinia pseudoacacia.
2Reliability
If single bacterial strain is used, then formulation simplicity is maintained, but synergistic effect on nodulation and nitrogen fixation is insufficient
Solution Approach 1:
The patent merges three different bacterial strains (Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28) into a single mixed bacteria formulation. This combination creates synergistic effects where Kocuria sp. X-22 promotes nodule formation, Microbacterium sp. X-26 enhances nitrogen fixation, and Bacillus sp. X-28 improves overall plant growth. The merged formulation achieves superior nodulation promotion compared to individual strains alone.
Solution Approach 2:
The patent develops a composite bacterial material consisting of three specifically selected strains that work together synergistically. This composite formulation integrates the complementary functions of each strain to reliably promote nodulation and nitrogen fixation in Robinia pseudoacacia, overcoming the limitations of single-strain preparations.
3Productivity
If spray seeding substrate is applied on rock surface, then greening of hard slopes is achieved, but substrate retention time is short
Solution Approach 1:
The patent employs bacteria (particularly Bacillus sp. X-28) that produce exopolysaccharides and other adhesive substances during their metabolic activities. These self-produced substances enhance the binding between the spray seeding substrate and the rock surface, allowing the substrate to anchor itself and retain moisture and nutrients for extended periods, thereby improving substrate retention time on hard slopes.
Solution Approach 2:
The patent utilizes readily available soil bacteria from Robinia pseudoacacia rhizosphere that can be cultured and applied in large quantities. These bacteria rapidly colonize the substrate and rock interface, providing immediate adhesive and protective functions that extend substrate retention, making the solution cost-effective and suitable for large-scale slope greening projects.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The mixed bacteria significantly increase the nodulation rate and nitrogen fixation level of Robinia pseudoacacia, promote photosynthesis, and enhance the growth and development of the plant, providing a synergistic additive effect and a new choice for growth-promoting bacteria in spray seeding substrates.
Implementation Method 1
soil microorganisms convert inert nitrogen in the air into ionic nitrogen that can be directly absorbed by vegetation
Implementation Method 2
the stomatal conductance of vegetation treated by microorganisms with a growth-promoting effect can be increased, which can accelerate the gas exchange of plant cells, and increase the intercellular CO2 concentration, so that the CO2 required for photosynthesis is sufficient, which further improves the photosynthetic rate of plants
Implementation Method 3
microorganisms can decompose incompatible minerals in the soil and degrade inorganic and organic pollutants during their life activities
Data Source
AI summary
A mixed bacteria for promoting nodulation and nitrogen fixation of Robinia pseudoacacia and their application are provided. The mixed bacteria includes Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28, all of which have been preserved in China Center for Type Culture Collection, and the preservation numbers respectively are: CCTCC No: M 2019237; CCTCC No: M 2019238; CCTCC No: M 2019239. The mixed bacteria are watered directly around the seedlings of Robinia pseudoacacia. Compared with the single bacteria control group and the sterile control group, the disclosure can produce synergistic superimposing effects, significantly improve the nodulation rate and symbiotic nitrogen fixation of the Robinia pseudoacacia, and promote the photosynthesis of the Robinia pseudoacacia.