Mixed Microbial Biocatalyst for CO2 Conversion

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Solution Overview

Problem

Current bioconversion methods for C1 feedstocks face challenges in product selectivity, energy efficiency, and resilience, particularly when using individual microbial strains, which are sensitive to contamination and operational parameters.

Innovation Solution

A mixed microbial community comprising bacteria from Peptostreptococcales-Tissierellales, Proteiniclasticum, Proteiniphilum, Mesobacillus, and Acetobacterium is used as a biocatalyst to convert C1 compounds into more desirable products, such as acetate and other organic acids, without the need for genetic modification or adjustment, operating at standard temperature and pressure.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If individual microbial strains are used for bioconversion, then high production rates can be achieved, but the system becomes sensitive to contamination and operational parameters

Engineering Contradiction:
Improveproduction rateVSAvoidresistance to contamination
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent combines multiple microbial strains into a mixed microbial community biocatalyst. This merging of different strains provides functional redundancy and resilience against contamination while maintaining high production rates for C1 compound conversion to acetate and other products.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The invention changes the fundamental parameter of the biocatalyst from a single strain to a mixed community composition. This parameter change transforms the system's reliability characteristics, making it more resistant to contamination and operational perturbations while preserving productivity.

Inventive Principle:
Principle #35Parameter changes

2Adaptability or versatility

If engineered pathways are used to expand product spectrum, then more diverse products can be produced, but the system complexity increases

Engineering Contradiction:
Improveproduct spectrumVSAvoidsystem complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent merges multiple microbial strains with different metabolic capabilities into a single mixed community biocatalyst. This natural merging provides diverse product spectrum including acetate, butyrate, caproate, lactate, ethanol, and other compounds without requiring complex genetic engineering of individual strains.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The mixed microbial community performs self-organization and self-regulation to achieve diverse product production. The community members naturally cooperate and compete to convert C1 compounds into various products, eliminating the need for complex external control systems or genetic modifications.

Inventive Principle:
Principle #25Self-service

3Productivity

If non-biological conversion routes are used, then direct CO2 reduction can be achieved, but high energy inputs and low product selectivity are required

Engineering Contradiction:
Improveconversion efficiencyVSAvoidenergy input
Core Design Contradiction:
ProductivityVSUse of energy by moving object

Solution Approach 1:

The patent replaces mechanical/thermal conversion systems with a biological system. Instead of using high temperature and pressure for direct CO2 reduction, the mixed microbial community biocatalyst performs conversion at ambient conditions, substituting biological catalysis for energy-intensive physical-chemical processes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The invention changes the operating parameters from high temperature and pressure (non-biological) to ambient temperature and pressure (biological). This parameter transformation enables efficient CO2 conversion while eliminating the need for high energy inputs, achieving both high productivity and low energy consumption.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach achieves high conversion rates of C1 compounds into desirable products, with over 80% conversion efficiency and stable operation, reducing sensitivity to feedstock content and environmental stressors, while generating significant yields of acetate and other valuable compounds.

Implementation Method 1

biological conversion exploits the natural ability of microorganisms to capture and utilize gaseous one-carbon (C1) compounds

Methodology Applied
Scientific EffectBiological conversion: Fermentation

Implementation Method 2

Bioconversion of gaseous and liquid C1 carbon substrates produces various acids, alcohols, and diols

Methodology Applied
Scientific EffectBioconversion: Fermentation

Data Source

PatentUS20240409968A1Enriched Microbial Biocatalyst for Conversion of CO2 into Acetate
Publication Date: 2024.12.12 THE UNITED STATES AS REPRESENTED BY THE DEPARTMENT OF ENERGY
  • US20240409968A1 patent drawing
  • US20240409968A1 patent drawing
  • US20240409968A1 patent drawing

AI summary

One or more embodiments relate to a method for converting a carbon feedstock into more desirable products involving contacting a biocatalyst with a carbon feedstock, where the biocatalyst converts the carbon feedstock into more desirable products, and where the biocatalyst features Peptostreptococcales-Tissierellales, Proteiniclasticum, Proteiniphilum, Mesobacillus, Acetobacterium, and combinations thereof.