Mobile Digital Spatial Profiling System Using UV Photocleaving
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Solution Overview
Problem
Current digital spatial profiling methods for biochemical characterization of pathological specimens are either high-cost, destructive, or lack spatial information, necessitating a more efficient and portable solution for imaging and analyzing tissue samples.
Innovation Solution
A compact, mobile digital spatial profiling system that uses UV light to cleave oligonucleotides from antibodies in specific regions of interest, allowing for the collection and hybridization of these oligonucleotides for quantitative analysis, integrated with a personal mobile device for imaging and data processing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional digital spatial profiling methods are used, then quantitative biochemical characterization is achieved, but the system becomes expensive, bulky, and complex
Solution Approach 1:
The system divides the digital spatial profiling function into separate modular components: a mobile device for imaging and data processing, and a separate UV cleaving chamber for the biochemical analysis. This segmentation allows each component to be optimized independently and reduces overall system complexity while maintaining measurement precision.
Solution Approach 2:
The patent introduces an intermediary UV light source that selectively cleaves oligonucleotides from antibodies in specific regions of interest. This intermediary mechanism enables the separation of imaging and biochemical analysis functions, allowing the system to achieve quantitative characterization without requiring a monolithic complex system.
2Measurement precision
If conventional digital spatial profiling methods are used, then biochemical characterization is achieved, but the system becomes destructive to tissue samples
Solution Approach 1:
The system applies local quality by using photomasking means to selectively illuminate only specific regions of interest with UV light. This localized approach ensures that only the desired tissue areas undergo photo-cleaving, while the rest of the tissue sample remains intact and available for further analysis or controls.
Solution Approach 2:
The method employs periodic UV illumination cycles where UV light is applied only after specific regions are identified and marked. This periodic action allows the system to perform biochemical characterization repeatedly on the same tissue sample without causing cumulative damage, as the UV exposure is controlled and timed precisely.
3Loss of information
If conventional digital spatial profiling methods are used, then spatial information is provided, but the system lacks portability
Solution Approach 1:
The patent merges the imaging, data processing, and spatial profiling functions into a single mobile device platform. By combining these functions in one portable unit, the system maintains the ability to provide comprehensive spatial information while achieving true portability, eliminating the need for separate bulky laboratory equipment.
Solution Approach 2:
The mobile device serves multiple functions: it captures images, processes data, provides spatial information, and coordinates the UV cleaving process. This multi-functionality consolidates what would traditionally require separate specialized equipment into a single portable system, maintaining spatial profiling capabilities while enabling field deployment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient, non-destructive, and spatially informative biochemical characterization of tissue samples, providing a lightweight and cost-effective alternative for disease state determination and etiology analysis.
Implementation Method 1
use UV light to cleave oligos (i.e., oligomer) off antibodies in one or more ROIs ('photo-cleaving')
Data Source
AI summary
Embodiments of the present disclosure are directed to a compact, mobile, digital spatial profiling (DSP) system, and associated apparatuses, devices and methods, which are configured to image one or more regions-of-interest (ROIs), and then using UV light to cleave, for example, oligos off antibodies in one or more ROIs (“photocleaving”), and collect the photocleaved oligos for later hybridization and counting.