Modified Activin A Proregion Protease Resistance

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Solution Overview

Problem

Conventional methods for producing activin A in plants face challenges due to degradation by proteases other than proprotein convertases, leading to reduced yields.

Innovation Solution

The amino acid sequence of the proregion of activin A is modified to create a modified activin A that is resistant to degradation by proteases other than proprotein convertases, specifically by deleting, substituting, or adding amino acids in the sequence from position 180 to 201.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If activin A is expressed in a plant using conventional methods, then production capability is achieved, but the expressed protein is degraded by endogenous plant proteases resulting in reduced yield

Engineering Contradiction:

Improveyield of activin AVSAvoiddegradation by protease

Core Design Contradiction:

ProductivityVSLoss of substance

Solution Approach 1:

The amino acid sequence of the proregion of activin A is modified by deleting, substituting, or adding amino acids at specific positions (particularly positions 180-201) to change the protein's resistance parameters against protease degradation while maintaining its biological function

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The modification is applied locally to the proregion of activin A rather than the entire protein structure, specifically targeting the amino acid sequence from position 180 to 201 to confer protease resistance without affecting other functional regions of the protein

Inventive Principle:

Principle #3Local quality

2Reliability

If the amino acid sequence of activin A is modified to resist protease degradation, then resistance to degradation is improved, but the complexity of the protein structure increases

Engineering Contradiction:

Improveresistance to protease degradationVSAvoidamino acid sequence complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The amino acid sequence is modified by relatively simple operations (deletion, substitution, or addition of amino acids) at specific positions rather than complex structural changes, thereby improving protease resistance with minimal increase in overall protein complexity

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS12291554B2Modified activin A

Publication Date: 2025.05.06 MITSUBISHI CHEM CORP

AI summary

The purpose of the present invention is to provide a modified activin A.The present invention provides activin A comprising a modified proregion.