Modified PIC9 Insecticidal Protein Expression Optimization
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Solution Overview
Problem
Current chemical and biotic insecticides used to control plant pests face limitations such as environmental contamination, drug-resistant insects, and high production costs, while transgenic plants expressing insect-resistant genes show promise but require improved expression levels and toxicity of insecticidal proteins like PIC9 for effective pest control.
Innovation Solution
Development of a modified PIC9 insecticidal protein and its gene encoding, optimized for high expression and toxicity in plants, specifically maize, through genetic engineering, involving recombinant vectors and expression cassettes, and co-expression with other insecticidal proteins to enhance pest resistance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If chemical insecticides are used to control plant pests, then pest control effectiveness is improved, but environmental contamination and drug-resistant insect appearance occur
Solution Approach 1:
The patent modifies the PIC9 protein sequence by changing specific amino acid residues (e.g., position 637 from Asn to Asp, positions 641-650 deletion) to optimize its expression and toxicity characteristics in transgenic plants, thereby improving pest control effectiveness while maintaining environmental safety
Solution Approach 2:
The patent creates composite insecticidal effects by co-expressing modified PIC9 protein with other insecticidal proteins (such as Cry1Ab) in the same transgenic plant, enhancing overall pest control effectiveness through synergistic or additive effects
2Object-affected harmful factors
If biotic insecticides are used to control plant pests, then environmental safety is improved, but production cost increases due to repeated application
Solution Approach 1:
The patent incorporates the modified PIC9 gene into the plant genome in advance, enabling the plant to continuously produce and accumulate the insecticidal protein throughout its growth cycle, eliminating the need for repeated applications and reducing production costs
Solution Approach 2:
The transgenic plant autonomously produces the modified PIC9 protein through its own metabolic systems, making the plant self-protective against pests without requiring external intervention or repeated insecticide applications
3Reliability
If PIC9 insecticidal protein is expressed in transgenic plants, then pest resistance is improved, but expression level and toxicity need enhancement for effective control
Solution Approach 1:
The patent optimizes the PIC9 gene sequence by modifying codon usage to match plant preference, removing introns, and adding regulatory elements (promoters, terminators) to maximize transcription and translation efficiency, thereby achieving high expression levels in transgenic plants
Solution Approach 2:
The patent uses tissue-specific or inducible promoters to drive PIC9 expression specifically in pest-damaged tissues or at critical growth stages, concentrating the insecticidal protein where it is most needed and enhancing overall effectiveness
Data Source
AI summary
The invention relates to an insecticidal protein, its gene encoding and the uses thereof. The protein comprises: (a) a protein consisting of an amino acid sequence shown by SEQ ID NO:2 or a sequence of amino acid residuses at positions 1 to 640 thereof; or (b) a protein derived from (a) by substitution, deletion, or addition of one or more amino acid residuses of the amino acid sequences in (a), and having insecticidal activity; or (c) a protein generated by the expression of nucleic acid molecules containing a sequence of amino acid residuses at the positions 1 to 1920 of SEQ ID NO:1; or (d) a protein generated by the expression of nucleic acid molecules containing a complementary sequence that hybridized with the sequence of nucleotides at the positions 1 to 1920 of SEQ ID NO:1 under stringent conditions. The protein has high expression level and strong toxicity against pests.