Modified PriL Gene Amino Acid Substitutions for Begomovirus Resistance
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Solution Overview
Problem
Current control measures against Begomoviruses, which affect economically valuable crop species in the Cucurbitaceae and Solanaceae families, are limited and primarily rely on cultural, phytosanitary, and chemical methods, with no well-characterized genetic basis for resistance.
Innovation Solution
A modified DNA Primase Large Subunit gene (PriL) with specific amino acid substitutions, such as phenylalanine to cysteine and glycine to aspartic acid, is introduced to confer resistance to Begomoviruses like ToLCNDV and CuLCV in plants, altering the interaction between viral replication proteins and host DNA Primase.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cultural, phytosanitary and chemical control measures are used against Begomoviruses, then whitefly transmission can be controlled, but these measures are limited and do not provide complete protection
Solution Approach 1:
The patent modifies the DNA Primase Large Subunit gene parameters by introducing specific amino acid substitutions (phenylalanine to cysteine at position 147, glycine to aspartic acid at position 162, glutamine to histidine at position 163) to alter the interaction between viral replication proteins and host DNA Primase, thereby providing genetic resistance that overcomes the limitations of external control measures
2Productivity
If the host cell replication machinery is used for viral replication, then Begomoviruses can multiply efficiently, but this makes the plant susceptible to infection
Solution Approach 1:
The patent converts the harmful dependency of viruses on host replication machinery into a benefit by modifying the host's DNA Primase Large Subunit gene. The modified PriL protein disrupts viral replication while maintaining essential host DNA replication functions, effectively turning the host's replication machinery from a vulnerability into a defense mechanism
Data Source
AI summary
The present invention relates to a modified PriL gene, which encodes a modified protein which may comprise one or more modifications in the wild type protein sequence of SEQ ID NO: 1 or SEQ ID NO: 3 or in a protein sequence having at least 60% sequence identity to SEQ ID NO: 1 or SEQ ID NO: 3. Alternatively, the wild type of the modified PriL gene encodes a protein according to SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, or SEQ ID NO: 31. The modification in the wild type protein sequence is an amino acid substitution.