Storage-Stable Liquid Dishwashing Detergent with Modified Protease
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Solution Overview
Problem
Protease- and amylase-containing liquid dishwashing detergents from prior art lack sufficient storage stability, leading to a significant loss of enzymatic activity, particularly amylolytic and proteolytic, resulting in reduced cleaning performance over time.
Innovation Solution
A liquid dishwashing detergent formulation that includes a protease with specific amino acid substitutions (L211 D, S3T, V4I, V193M, and V199I) combined with an amylase, which maintains improved stability and cleaning performance even after storage at elevated temperatures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If liquid dishwashing detergent contains protease and amylase, then cleaning performance is improved, but storage stability deteriorates and enzymatic activity is lost over time
Solution Approach 1:
The invention modifies the amino acid sequence of the protease by introducing specific substitutions (S3T, V4I, V193M, V199I, L211D) to change the enzyme's properties. These parameter changes in the protease structure improve storage stability and reduce inactivation of amylase while maintaining cleaning performance.
2Reliability
If liquid dishwashing detergent contains protease, then proteolytic cleaning performance is improved, but amylolytic activity is inactivated and lost
Solution Approach 1:
The invention converts the harmful effect of protease on amylase into a beneficial outcome. By modifying the protease with specific amino acid substitutions, the harmful inactivation of amylase is eliminated, allowing both enzymes to coexist and function effectively together in the detergent formulation.
Solution Approach 2:
The amino acid substitutions in the protease structure change its interaction properties with amylase, preventing inactivation while preserving proteolytic activity. This parameter change resolves the conflict between the two enzymes.
3Reliability
If liquid dishwashing detergent contains conventional protease variants, then proteolytic activity is maintained, but storage stability and amylase compatibility are insufficient
Solution Approach 1:
The invention creates a composite enzyme system where a modified protease and amylase work together in the detergent formulation. The specific amino acid substitutions in the protease enable compatible interaction with amylase, creating a synergistic enzyme combination that maintains both proteolytic and amylolytic activities.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The detergent exhibits enhanced storage stability and cleaning performance on protease-sensitive soils, maintaining enzymatic activity and effectiveness for extended periods, including after storage for four weeks at 40 °C.
Implementation Method 1
a protease comprising an amino acid sequence corresponding to that in SEQ ID NO: 1 specified amino acid sequence over whose total length at least 71% is identical and in the count according to SEQ ID NO: 1 the amino acid substitution L211 D in combination with the amino acid substitutions S3T, V4I, V193M and V199I
Implementation Method 2
The proteases used in dishwashing detergents known from the prior art either originate from microorganisms... Subtilisin-type proteases are preferably used in dishwashing detergents
Implementation Method 3
An amylase is an enzyme that catalyzes the hydrolysis of glycoside bonds, especially in polysaccharides such as starch
Implementation Method 4
an amylase... Amylases therefore work particularly against starch-containing residues in laundry and catalyze their hydrolysis
Data Source
AI summary
The aim of the invention is to improve the storage stability of a liquid dishwashing detergent comprising a protease and an amylase. Said aim is achieved by using a protease that has an amino-acid sequence that is at least 70% identical to the amino-acid sequence specified in SEQ ID NO. 1 over the entire length of the amino-acid sequence and has the amino-acid substitution L211D in combination with at least two additional amino-acid substitutions selected from the group comprising S3T, V4I, V193M, and V199I in the count as per SEQ ID NO. 1.