Modified Protease Inhibitor for Detergent Reactivation
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Solution Overview
Problem
Existing proteinaceous protease inhibitors either inhibit proteases too strongly, preventing their reactivation, or fail to maintain protease activity over long-term storage in detergent compositions.
Innovation Solution
Development of protease inhibitors with specific amino acid sequence modifications that allow for moderate inhibitory activity and reactivation by dilution, maintaining protease activity in protein solutions and detergent compositions even after long-term storage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If known naturally occurring protease inhibitors are used, then protease activity is strongly inhibited, but the proteases cannot be reactivated by dilution
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the protease inhibitor to alter its inhibitory strength. Specifically, the inhibitor is designed with reduced binding affinity to the protease active site, allowing it to inhibit protease activity during storage while permitting reactivation through dilution. This is achieved by changing parameters such as the inhibitor's molecular structure and interaction strength with the protease.
2Reliability
If protease inhibitors with strong inhibitory activity are used, then protease self-hydrolysis is prevented, but cleaning performance is lost over time
Solution Approach 1:
The patent applies dynamics by creating a dynamic inhibition system where the inhibitor's effect can be modulated. During storage, the inhibitor maintains protease stability by preventing self-hydrolysis. During use, dilution reduces the inhibitor concentration, allowing protease activity to be restored for effective cleaning. This dynamic adjustment enables the system to switch between stability and activity modes.
Solution Approach 2:
The patent applies preliminary action by pre-added protease inhibitors that protect the protease during storage before use. The inhibitor is introduced in advance to prevent protease degradation, and the protective effect is temporarily maintained until dilution occurs at the point of use, at which point the protease becomes active for cleaning.
3Stability of the object's composition
If conventional protease inhibitors are used in detergent compositions, then protease activity is maintained during storage, but the solution cannot be reactivated for use
Solution Approach 1:
The patent modifies the physical-chemical parameters of the protease inhibitor, specifically its amino acid sequence and molecular properties, to achieve moderate inhibitory strength. This parameter adjustment allows the inhibitor to maintain protease activity during storage while enabling reactivation through simple dilution, improving ease of operation without compromising stability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified protease inhibitors effectively inhibit protease activity while allowing for reactivation and maintaining cleaning performance in detergent compositions over time, ensuring stable protease activity and cleaning efficacy.
Implementation Method 1
a proteinaceous protease inhibitor... which inhibits the activities of proteases, and allows the proteases to be reactivated
Data Source
AI summary
An object of the present invention is to provide proteinaceous protease inhibitors with moderate inhibitory activity. A proteinaceous protease inhibitor of the present invention includes an amino acid sequence (Y) or an amino acid sequence (Y'), the amino acid sequence (Y) being different from the amino acid sequence (A) of a proteinaceous protease inhibitor (BC), represented by SEQ ID NO:1, by one to eight amino acid replacements with amino acids different from replaced amino acids, the amino acid sequence (Y') having at least 80% homology to the amino acid sequence (Y), wherein the proteinaceous protease inhibitor satisfies at least one of the following conditions (1) to (8) : (1) the amino acid residue at a position corresponding to residue 12 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X1) defined below; (2) the amino acid residue at a position corresponding to residue 38 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X2) defined below; (3) the amino acid residue at a position corresponding to residue 48 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X3) defined below; (4) the amino acid residue at a position corresponding to residue 50 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X4) defined below; (5) the amino acid residue at a position corresponding to residue 51 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X5) defined below; (6) the amino acid residue at a position corresponding to residue 52 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X6) defined below; (7) the amino acid residue at a position corresponding to residue 53 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X7) defined below; (8) the amino acid residue at a position corresponding to residue 70 of the amino acid sequence (A) in the amino acid sequence (Y) or (Y') is an amino acid (X8) defined below: (X1): any of amino acids (X0) other than Glu (X2): any of the amino acids (X0) other than Val and Ile (X3): any of the amino acids (X0) other than Met (X4): any of the amino acids (X0) other than Tyr (X5): any of the amino acids (X0) other than Arg (X6): any of the amino acids (X0) other than Ile (X7): any of the amino acids (X0) other than Asp (X8): any of the amino acids (X0) other than Arg; and (X0): Ala, Arg, Asn, Asp, Cys, Gly, Gln, Glu, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.