Modified Xylanase Polypeptide for Thermal and pH Stability
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Most xylanases exhibit low pH and thermal stability, limiting their application in various industrial fields.
Innovation Solution
A modified polypeptide with xylanase activity, featuring specific amino acid substitutions and disulfide bond formations, enhancing thermal tolerance and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If xylanase is used in harsh conditions (high temperature, basic conditions), then industrial application capability is improved, but most xylanases have low thermal stability and low pH conditions limiting their use
Solution Approach 1:
The patent applies parameter changes by modifying specific amino acid residues at positions 3, 5, 20, 34, 36, and 41 to alter the thermal and pH stability parameters of the xylanase enzyme, enabling it to function reliably in harsh industrial conditions while maintaining its catalytic activity
Solution Approach 2:
The patent creates a composite structure by introducing disulfide bonds between cysteine residues at specific positions, forming a more stable composite protein structure that resists denaturation at high temperatures and extreme pH levels, thereby improving both thermal stability and adaptability
2Reliability
If amino acid substitutions and disulfide bond formations are introduced to enhance thermal tolerance, then thermal stability is improved, but protein structure complexity increases
Solution Approach 1:
The patent applies local quality by making targeted amino acid substitutions at specific positions (3, 5, 20, 34, 36, and 41) rather than throughout the entire protein structure, and by forming disulfide bonds at specific locations, thereby improving thermal stability with minimal increase in overall structural complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified polypeptide demonstrates improved thermal tolerance and stability, enabling its effective use in diverse industrial applications.
Implementation Method 1
Xylanase (EC 3.2.1.8) is a hydrolase that randomly degrades the β-1,4 backbone of xylan
Data Source
AI summary
The present disclosure relates to a modified polypeptide having xylanase activity and the use thereof.