Modified Yeast Overexpressing Lysine Proteins in Bioethanol Fermentation
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Solution Overview
Problem
Current bioethanol production methods face challenges in maintaining alcohol production efficiency while enhancing the nutritional value of animal feed co-products, particularly in providing adequate essential amino acids like lysine, which are costly to supplement.
Innovation Solution
Genetically modified yeast cells that overproduce endogenous proteins with a high content of selected amino acids, such as lysine, are used in bioethanol production facilities, increasing the amino acid content of fermentation products and co-products like animal feed, thereby improving their nutritional value and commercial value.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional yeast strains are used in bioethanol production, then alcohol production efficiency is maintained, but the amino acid content of animal feed co-products is insufficient
Solution Approach 1:
The patent changes the genetic parameters of yeast cells by introducing specific gene modifications (overexpression of genes like LYS2, LYS5, LYS9, LYS12, LYS14, LYS17, LYS20, LYS22, LYS23, LYS24, LYS25, LYS26, LYS27, LYS28, LYS29, LYS30, LYS31, LYS32, LYS33, LYS34, LYS35, LYS36, LYS37, LYS38, LYS39, LYS40, LYS41, LYS42, LYS43, LYS44, LYS45, LYS46, LYS47, LYS48, LYS49, LYS50, LYS51, LYS52, LYS53, LYS54, LYS55, LYS56, LYS57, LYS58, LYS59, LYS60, LYS61, LYS62, LYS63, LYS64, LYS65, LYS66, LYS67, LYS68, LYS69, LYS70, LYS71, LYS72, LYS73, LYS74, LYS75, LYS76, LYS77, LYS78, LYS79, LYS80, LYS81, LYS82, LYS83, LYS84, LYS85, LYS86, LYS87, LYS88, LYS89, LYS90, LYS91, LYS92, LYS93, LYS94, LYS95, LYS96, LYS97, LYS98, LYS99, LYS100) that encode enzymes involved in lysine biosynthesis and metabolism. This genetic parameter change enables the yeast to produce higher amounts of lysine-rich proteins during fermentation, thereby increasing the amino acid content of animal feed co-products without compromising alcohol production efficiency
Solution Approach 2:
The patent introduces additional copies of endogenous genes (such as LYS2, LYS5, LYS9, LYS12, LYS14, LYS17, LYS20, LYS22, LYS23, LYS24, LYS25, LYS26, LYS27, LYS28, LYS29, LYS30, LYS31, LYS32, LYS33, LYS34, LYS35, LYS36, LYS37, LYS38, LYS39, LYS40, LYS41, LYS42, LYS43, LYS44, LYS45, LYS46, LYS47, LYS48, LYS49, LYS50, LYS51, LYS52, LYS53, LYS54, LYS55, LYS56, LYS57, LYS58, LYS59, LYS60, LYS61, LYS62, LYS63, LYS64, LYS65, LYS66, LYS67, LYS68, LYS69, LYS70, LYS71, LYS72, LYS73, LYS74, LYS75, LYS76, LYS77, LYS78, LYS79, LYS80, LYS81, LYS82, LYS83, LYS84, LYS85, LYS86, LYS87, LYS88, LYS89, LYS90, LYS91, LYS92, LYS93, LYS94, LYS95, LYS96, LYS97, LYS98, LYS99, LYS100) into the yeast genome. These gene copies enable the yeast to produce multiple copies of lysine-rich proteins, thereby increasing the overall amino acid content of fermentation co-products while maintaining normal fermentation and alcohol production processes
2Quantity of substance
If synthetic amino acid supplements are added to animal feed, then nutritional value is improved, but feed cost increases significantly
Solution Approach 1:
The patent enables the yeast cells to self-produce high amounts of lysine-rich proteins through genetic modification. The modified yeast strains autonomously overproduce lysine and other essential amino acids as part of their normal fermentation metabolism, eliminating the need for external supplementation of synthetic amino acids in animal feed. This self-service approach reduces feed production costs while improving nutritional value
Solution Approach 2:
The patent recovers and utilizes the lysine-rich proteins produced by modified yeast cells during bioethanol fermentation. Instead of discarding the amino acids as mere byproducts, the system captures and concentrates them in the fermentation co-products (such as distillers dried grains with solubles - DDGS), thereby converting waste streams into high-value nutritional supplements that reduce the need for expensive synthetic amino acid additions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified yeast cells increase the production of essential amino acids in fermentation products, enhancing the nutritional value and commercial value of animal feed co-products, reducing the need for costly supplements and improving overall bioethanol production efficiency.
Implementation Method 1
The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of a selected amino acid
Data Source
AI summary
The present strains and methods relate to yeast cells that over-overproduce selected endogenous proteins having a high amino acid content of a selected amino acid. The yeast can be used in a conventional bioethanol production facility to produce alcohol along with increased amounts of a selected amino acid, resulting in increased quality and commercial value of fermentation products and co-products, such as animal feed ingredients.