Modular Nucleic Acid Tags for Universal Sequencing Library Prep
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Solution Overview
Problem
Existing nucleic acid sequencing workflows face limitations as they are often specific to particular sample types and sequencing systems, lacking universality and compatibility.
Innovation Solution
A method for preparing a library of nucleic acids with modular end sequences involves combining a pool of different modular nucleic acid tags with a nucleic acid sample, joining the ends of target nucleic acids to these tags, amplifying the tagged nucleic acids, and detecting the amplified library.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If existing nucleic acid sequencing workflows are used, then sample preparation is specific to particular sample types and sequencing systems, but this limits universality and compatibility across different platforms
Solution Approach 1:
The patent applies universality by designing a standardized library preparation workflow with modular components that can be used across different sequencing platforms (Illumina, Ion Torrent, PacBio, Oxford Nanopore). The method uses universal adapters and a standardized protocol that works with various sample types (genomic DNA, cDNA, amplicons) and sequencing systems, eliminating the need for platform-specific optimization while maintaining high compatibility
Solution Approach 2:
The patent applies segmentation by dividing the library preparation process into distinct modular steps: fragmentation, end repair, adapter ligation, and amplification. Each step uses standardized reagents and conditions that can be independently optimized and combined, allowing the workflow to be adapted to different sequencing platforms without redesigning the entire process, thus reducing overall complexity while improving versatility
2Adaptability or versatility
If standardized modular tags are used for all samples, then compatibility across sequencing systems is improved, but the ability to handle sample-specific requirements may be reduced
Solution Approach 1:
The patent applies local quality by allowing customization of specific workflow parameters and adapter sequences for particular sample types or sequencing platforms while maintaining the overall standardized framework. For example, fragmentation conditions, adapter sequences, or amplification cycles can be locally optimized for specific applications (e.g., FFPE samples, low-input samples) without compromising the universal compatibility of the core workflow
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the preparation of nucleic acid libraries that are compatible with a wide range of sample types and sequencing systems, facilitating universal applicability and improving sequencing efficiency.
Implementation Method 1
amplifying each of the doubly-tagged target nucleic acids
Implementation Method 2
joining the ends of each of the double-stranded target nucleic acids to tags selected from the pool of different modular nucleic acid tags
Data Source
AI summary
The present disclosure provides a method of preparing a library of nucleic acids having modular end sequences. The method includes combining a pool of different modular nucleic acid tags with a nucleic acid sample, the nucleic acid sample including a plurality of double-stranded target nucleic acids. The method further includes joining the ends of each of the double-stranded target nucleic acids to tags selected from the pool of different modular nucleic acid tags to form a plurality of doubly-tagged target nucleic acids, amplifying each of the doubly-tagged target nucleic acids, thereby preparing a library of nucleic acids having modular end sequences, and detecting the library of amplified nucleic acids having modular end sequences.