MOFs-Coated Cardiomyocyte Core-Shell Structure
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Solution Overview
Problem
Bio-hybrid microrobots face challenges due to lack of physical protection and nutrient supply channels, leading to short life cycles and limited understanding of material design at the molecular level.
Innovation Solution
A method for preparing a metal-organic frameworks (MOFs)-coated cardiomyocyte core-shell structure is developed, where cardiomyocytes are coated with an MOFs layer through biomimetic mineralization, enabling selective penetration of nutrients and gases, thereby enhancing cell survival and longevity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cardiomyocytes are used as drivers for bio-hybrid microrobots, then spontaneous contraction and rhythmic movement are achieved, but physical protection and nutrient supply are insufficient
Solution Approach 1:
The patent applies this principle by coating cardiomyocytes with a MOF thin film shell that provides physical protection while maintaining flexibility. The MOF layer acts as a protective shell that shields cells from environmental damage while allowing necessary exchanges, directly resolving the contradiction between protection and cell viability.
Solution Approach 2:
The patent utilizes the porous structure of MOF materials to create a coating that allows selective penetration of nutrients and gases. The controlled porosity enables beneficial substances to reach cells while blocking harmful factors, thus improving cell survival without compromising nutrient supply.
2Duration of action of stationary object
If MOFs are coated on cardiomyocytes to provide physical protection, then cell lifespan is extended, but nutrient and gas penetration must be maintained
Solution Approach 1:
The MOF coating's porous structure enables selective transport of nutrients and gases while providing protection. The porosity allows essential substances to penetrate to the cells, extending cell lifespan without compromising nutrient supply.
Solution Approach 2:
The patent creates a composite structure combining MOF inorganic framework with organic biological components. This composite material provides both protective functions and selective permeability, allowing the coating to extend cell lifespan while maintaining nutrient and gas penetration.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The MOFs layer provides physical protection and ensures the necessary nutrients and gases reach the cells, significantly increasing cell density and prolonging the lifespan of cardiomyocytes, as demonstrated by higher cell density after 7 days of culture compared to uncoated cardiomyocytes.
Implementation Method 1
The present disclosure aims to in situ construct an MOFs physical protection layer on the surface of a biological material by means of biomimetic mineralization
Implementation Method 2
The MOFs physical protection layer can selectively penetrate the nutrients and gases necessary for the survival of the biological material
Implementation Method 3
preparing a Zn(NO3)2·6H2O aqueous solution and a 2-methylimidazole aqueous solution... to obtain the MOFs-coated cardiomyocyte core-shell structure
Data Source
AI summary
The present disclosure provides a preparation method of an MOFs-coated cardiomyocyte core-shell structure, and relates to a method for coating cardiomyocytes. The present disclosure aims to solve problems that bio-hybrid microrobots in the prior art lack physical protection and nutrient supply channels and have short life cycles. The method includes the steps of: 1) cleaning; 2) adding trypsin in PBS; 3) digesting; 4) centrifuging; 5) culturing; 6) passaging; 7) preparing a Zn(NO3)2·6H2O aqueous solution and a 2-methylimidazole aqueous solution; and 8) adding the Zn(NO3)2·6H2O aqueous solution and the 2-methylimidazole aqueous solution for culture to obtain the MOFs-coated cardiomyocyte core-shell structure. After 7 days of culture under the same culture conditions, cardiomyocytes having an MOFs shell layer have significantly higher cell density than the cardiomyocytes. An MOFs-coated cardiomyocyte core-shell structure can be obtained according to the present disclosure.