Molecular Array Analysis Method for High-Throughput Screening
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for screening and optimizing biochemical processes and molecular properties are time-consuming, labor-intensive, and costly, often leading to biases that result in the suppression of optimal molecules with desired properties due to side properties, and are limited in their ability to analyze large numbers of molecules simultaneously with high parallelism and purity.
Innovation Solution
A method involving the use of a first memory with a defined arrangement of sample molecules, followed by the creation of transfer memories through transfer reactions, amplification, or derivatization, allowing for the spatial separation and analysis of multiple copies of molecules, enabling simultaneous analysis of molecular structure and properties with high purity and throughput.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If combinatorial production methods are used to generate large molecular libraries, then the quantity of molecules that can be produced increases, but the ability to determine which specific molecule generates a signal is lost due to mixing
Solution Approach 1:
The invention divides the molecular library into separate individual wells, with each well containing a specific molecule or mixture. This segmentation allows parallel processing of many molecules while maintaining the ability to identify which specific well produced a signal, thus preserving molecular structure identification information.
Solution Approach 2:
The invention creates copies of molecular libraries on solid supports (microtiter plates, microarrays), where each position on the support corresponds to a specific molecule or mixture. This copying approach enables simultaneous analysis of many molecules while maintaining spatial information about which molecule generated which signal.
2Measurement precision
If individual production and measurement of each molecule is performed, then molecular structure identification is accurate, but the throughput and productivity are extremely limited
Solution Approach 1:
The invention combines individual molecule placement with parallel processing by arranging many individual molecular samples in separate wells or positions on a solid support. This allows simultaneous measurement of numerous molecules (high productivity) while maintaining the ability to identify each molecule's structure (high precision) through its specific position or barcode identifier.
3Quantity of substance
If repeated selection steps are performed to enrich desired molecules, then the concentration of target molecules increases, but selection bias suppresses optimal molecules with undesired side properties
Solution Approach 1:
The invention performs preliminary characterization of all molecules in the library before selection, by measuring properties such as binding affinity, enzymatic activity, or other desired properties for every molecule in parallel. This preliminary data allows for unbiased identification of optimal molecules without requiring repeated selection steps that would introduce bias against molecules with undesired side properties.
4Ease of operation
If the pool of molecules is restricted to a manageable number for individual testing, then the analysis becomes feasible, but the diversity and number of candidates examined decreases
Solution Approach 1:
The invention replaces manual or sequential mechanical processing of individual molecules with automated high-throughput systems. Using robotic liquid handlers, automated plate readers, and microfluidic devices, the system can process thousands of individual molecular samples in parallel, making individual testing of large numbers of candidates both feasible and efficient.
Data Source
AI summary
The invention relates to a method for analyzing molecular properties and/or reaction conditions, comprising a step of providing a first store having a first surface, wherein a specific selection of sample molecules is directly or indirectly bonded to the surface in a defined arrangement, a step of producing at least two transfer stores, wherein at least two additional surfaces are provided, and a reaction step, selected from the group comprising a transfer reaction, an amplification reaction, and/or a derivatization reaction, whereby product molecules can arise and said product molecules and/or the sample molecules bond to the surfaces, wherein there is a clear spatial association between the sample molecules of the first store and the product molecules and/or sample molecules of the transfer stores and the first store, the transfer stores, the sample molecules, the product molecules, the transfer reaction, the amplification reaction, and/or the derivatization reaction is analyzed.