Combinatorial Molecular Library Generation via Segmented Barcode Coupling
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Solution Overview
Problem
Current methods lack efficient systems for generating diverse libraries of multiple types of molecules, such as nucleic acid and peptide molecules, which are essential for characterizing biological samples and identifying components like cells.
Innovation Solution
A method involving the combinatorial generation of molecules on supports like beads, where different types of molecules are sequentially coupled and partitioned into separate environments, allowing for the creation of libraries with unique barcode sequences for identification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If combinatorial generation is used to create large sets of molecules, then the quantity of molecules is improved, but the complexity of managing multiple molecule types deteriorates
Solution Approach 1:
The patent segments the generation process into distinct stages: first generating nucleic acid molecules with barcodes, then separately generating peptide molecules, and finally coupling them together. This segmentation allows each molecule type to be generated and managed independently, reducing the overall complexity while maintaining the ability to create large combinatorial libraries
Solution Approach 2:
The patent uses barcoded nucleic acid molecules as intermediaries that serve dual purposes: they act as identifiers and as coupling partners for peptide molecules. This intermediary approach enables the systematic tracking and management of multiple molecule types through the barcode system, simplifying the coordination complexity
2Adaptability or versatility
If multiple types of molecules are generated together, then the versatility of the molecular library is improved, but the difficulty of identification and characterization deteriorates
Solution Approach 1:
The patent creates a informational copy of each peptide molecule in the form of a complementary barcoded nucleic acid sequence. This barcode copy serves as a readable record that enables easy identification and characterization of the peptide without requiring direct analysis of the peptide itself, thus reducing the difficulty of detecting and measuring peptide properties
Solution Approach 2:
The patent transforms the identification problem from direct peptide analysis to nucleic acid sequence reading by changing the detection parameter from peptide-specific assays to universal nucleic acid sequencing. This parameter change simplifies identification while maintaining versatility across different peptide types
Data Source
AI summary
The present disclosure provides methods and systems for generation of compositions comprising two or more sets of molecules. Compositions herein may comprise sets of molecules of different types. Sets of molecules may be generated by attachment to supports. Different types of molecules may be used to generate a large number of unique molecules. One or more types of molecules may be used to identify one or more additional types of molecules. Compositions of the present disclosure may be used in, for example, nucleic acid sequencing.