Molecular Net 3D Matrix for Analyte Capture
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Solution Overview
Problem
Current analyte capture technologies face limitations in sensitivity, specificity, signal-to-noise ratio, and cost, particularly in capturing analytes from complex samples with minimal sample preparation and achieving effective measurement and detection.
Innovation Solution
The development of a solid phase device with a molecular net comprising capture molecules linked by various types of linker molecules to form a covalently-linked multi-layered three-dimensional matrix, which can selectively capture analytes and enhance detection by positioning them for optimal measurement.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a single layer of capture molecules is used for analyte capture, then the device structure is simple, but the sensitivity and signal-to-noise ratio are limited
Solution Approach 1:
The patent transitions from a two-dimensional single layer of capture molecules to a three-dimensional molecular net structure. This dimensional change increases the capture capacity and signal intensity while maintaining structural organization through the network architecture of interconnected capture molecules and linker molecules.
Solution Approach 2:
The molecular net is constructed as a composite structure comprising capture molecules of at least one type and linker molecules of a plurality of types covalently bonded to form a multi-layered three-dimensional matrix. This composite architecture enhances sensitivity and signal-to-noise ratio compared to simple single-layer structures.
2Quantity of substance
If capture molecules are densely packed on the surface, then the capture capacity increases, but non-specific binding increases
Solution Approach 1:
The molecular net structure creates localized capture zones where capture molecules are positioned at specific nodes within the three-dimensional network. This local organization maintains high capture capacity while the distributed network architecture reduces non-specific binding by preventing uniform surface coverage that promotes non-specific interactions.
Solution Approach 2:
The covalently-linked multi-layered three-dimensional matrix forms a porous network structure with interconnected voids and channels. This porous architecture increases capture capacity by providing multiple access pathways and binding sites while reducing non-specific binding through size exclusion and reduced surface contact area.
3Loss of time
If sample preparation is minimized for rapid detection, then the analysis time is reduced, but the complexity of handling unprocessed samples increases
Solution Approach 1:
The molecular net structure enables direct capture of analytes from unprocessed samples without requiring extensive sample preparation steps. The three-dimensional network with its multiple binding sites and porous structure performs self-service by automatically capturing target analytes while excluding interfering substances, eliminating the need for complex pretreatment protocols.
Solution Approach 2:
The patent changes the structural parameters of the capture surface from simple two-dimensional layers to complex three-dimensional molecular nets with controlled porosity and connectivity. This parameter change allows the system to handle unprocessed samples directly by providing steric exclusion of interferents while maintaining high affinity capture of target analytes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables efficient, sensitive, and specific analyte capture and measurement with reduced non-specific binding, improved signal intensity, and cost-effectiveness, allowing for real-time detection and purification from unprocessed samples.
Implementation Method 1
The capture molecules may be configured to bind to the analyte
Data Source
AI summary
Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a Molecular Net) for specific and sensitive analyte capture from a sample. Also disclosed herein is a Molecular Net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample. A Molecular Net may comprise a pseudorandom nature. Use of various capture molecules, linkers and spacers in a Molecular Net may confer unique binding properties to a Molecular Net. Porosity, binding affinity, size exclusion abilities, filtration abilities, concentration abilities and signal amplification abilities of a Molecular Net may be varied and depend on the nature of components used in its fabrication. Uses of a Molecular Net may include analyte capture, analyte enrichment, analyte purification, analyte detection, analyte measurement and analyte delivery. Molecular Nets may be used in liquid phase or on solid phases such as nanomaterials, modified metal surfaces, nanospheres, microspheres, microtiter plates, slides, pipettes, cassettes, cartridges, discs, probes, lateral flow devices, microfluidics devices, microfluidics devices, optical fibers and others.