MON 87411 Corn Event With Linked Cassettes for Rootworm and Glyphosate
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Solution Overview
Problem
Existing transgenic corn plants exhibit variability in insect resistance and herbicide tolerance due to factors like chromosomal insertion site, expression cassette orientation, and environmental conditions, making it difficult to predict and achieve desirable phenotypic traits consistently.
Innovation Solution
The development of transgenic corn event MON 87411, which incorporates three linked expression cassettes providing dual modes of action against corn rootworms and tolerance to glyphosate, utilizing specific DNA segments for detection and characterized by unique nucleotide sequences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple transgenes are introduced to provide multiple traits (insect resistance and herbicide tolerance), then the functionality and versatility of the plant is improved, but the device complexity and difficulty of detection increase
Solution Approach 1:
The patent combines multiple transgenes (Cry3Bb for rootworm resistance and CP4 EPSPS for glyphosate tolerance) into a single integration event at one chromosomal locus. This merging approach maintains multiple traits while simplifying the overall event structure compared to having separate integration events for each trait.
Solution Approach 2:
The single integration event serves multiple functions simultaneously: it provides both insect resistance and herbicide tolerance traits, and it creates a unified molecular target for detection. The event structure is designed to be universally applicable across different corn varieties through standard breeding procedures.
2Reliability
If extensive molecular characterization and field trials are conducted to select superior events, then the reliability and manufacturing precision are improved, but the loss of time and productivity decrease
Solution Approach 1:
The patent performs preliminary molecular characterization during the event generation process itself, identifying unique diagnostic sequences before field trials begin. This preliminary action allows for early elimination of unsuitable events, reducing the number of events that require extensive field testing and thereby reducing overall development time.
Solution Approach 2:
The patent creates diagnostic molecular copies (unique DNA sequences) that can be used to rapidly identify and track the desired event through breeding generations. These molecular copies serve as surrogates for phenotypic assessment, allowing faster selection compared to waiting for field trial results alone.
3Adaptability or versatility
If transgenes are inserted at various chromosomal locations to generate multiple events, then the adaptability and versatility are improved, but the device complexity and measurement precision requirements increase
Solution Approach 1:
The patent identifies specific local characteristics (unique flanking sequences) at the insertion site that are diagnostic for the desired event. Rather than characterizing the entire genome, the focus is on the local region surrounding the insertion site, reducing the measurement precision requirements while still enabling accurate event identification.
Solution Approach 2:
The patent uses unique flanking sequences as intermediary markers that link the chromosomal insertion site to the transgene. These intermediary sequences facilitate easy detection and tracking of the event without requiring direct characterization of the insertion mechanism or entire genomic context.
Data Source
AI summary
The invention provides corn event MON 87411, and plants, plant cells, seeds, plant parts, and commodity products comprising event MON 87411. The invention also provides polynucleotides specific for event MON 87411 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 87411. The invention also provides methods related to event MON 87411.