MON87712 Transgenic Soybean Yield Detection
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Solution Overview
Problem
Current methods for increasing soybean yield through transgenic plants involve significant variability in transgene expression, requiring the screening of hundreds of transformation events to identify suitable traits, and there is a need for efficient detection methods to identify and verify the presence of specific transgenic events in soybean crops.
Innovation Solution
The development of transgenic soybean event MON87712, which includes specific DNA molecules and methods for detecting these molecules using nucleotide sequences and primers, allowing for the identification and verification of the event in soybean plants and products, thereby facilitating the production of high-yield soybean varieties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If transgenic plants are used to increase soybean yield, then yield improvement is achieved, but significant variability in transgene expression occurs requiring screening of hundreds of transformation events
Solution Approach 1:
The patent modifies key parameters of the transgene expression system including using the CaMV 35S promoter for constitutive high-level expression, optimizing the coding sequence of the yield-related gene, and adjusting regulatory elements to achieve consistent high expression across transformation events, thereby reducing the need to screen hundreds of events
2Reliability
If hundreds of transformation events are screened to identify suitable traits, then reliable transgene expression is achieved, but time and resource consumption increases significantly
Solution Approach 1:
The patent performs preliminary optimization of the transgene construct design before transformation, including selecting proven promoters, optimizing gene sequences for the target organism, and pre-testing expression levels in model systems. This preliminary action ensures that most transformation events produce reliable expression, dramatically reducing the number of events requiring extensive screening
3Measurement precision
If detection methods are developed to identify specific transgenic events, then verification accuracy is improved, but method complexity increases
Solution Approach 1:
The patent extracts and utilizes unique diagnostic DNA sequences from the transgene construct and its integration site into the soybean genome. By designing detection primers and probes that target these unique junction sequences, the method achieves high verification accuracy while maintaining simplicity, as it only requires standard PCR and sequencing techniques without complex instrumentation
Data Source
AI summary
The present invention provides a transgenic soybean comprising event MON87712 that exhibits increased yield. The invention also provides cells, plant parts, seeds, plants, commodity products related to the event, and DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a soybean plant. The invention further provides methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event.