MON87751 Soybean Event With Dual Toxin Cassettes for Durable Pest Control
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Solution Overview
Problem
Existing transgenic soybean plants rely on a single toxin for insecticidal control, which can lead to limited durability due to the increased likelihood of insect resistance development, and those with multiple toxins may not effectively target different receptors in the insect midgut, compromising durability.
Innovation Solution
A transgenic soybean event (MON87751) is developed with two linked expression cassettes that encode Cry2Ab and Cry1A.105 proteins, providing dual modes of action against lepidopteran pests, ensuring redundancy in insecticidal control and reducing resistance development.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a single toxin is used for insecticidal control, then the plant structure is simpler and easier to manufacture, but the durability is reduced due to increased likelihood of insect resistance development
Solution Approach 1:
The invention divides the insecticidal control function into two separate toxin components (Cry1Ac and Cry1F) that target different insect receptors. This segmentation allows each toxin to independently control specific pest populations, reducing the likelihood of resistance development while maintaining manageable plant structure through modular gene expression cassettes.
Solution Approach 2:
The plant expresses a composite insecticidal system combining two different Bt toxin proteins (Cry1Ac and Cry1F) with distinct modes of action. This composite approach creates synergistic pest control by targeting multiple insect receptors simultaneously, enhancing durability without significantly complicating the manufacturing process through standardized transgenic expression systems.
2Reliability
If multiple toxins are used for insecticidal control, then the durability is improved by reducing resistance development, but the device complexity increases
Solution Approach 1:
The plant uses a universal expression cassette system that can accommodate multiple toxin genes (Cry1Ac and Cry1F) under standardized promoter and regulatory elements. This multi-functional approach allows the same genetic architecture to express different toxin combinations, managing complexity through reusable molecular templates while maintaining enhanced durability through diverse pest targeting.
3Device complexity
If multiple toxins are used that target the same receptors, then the device complexity is reduced, but the durability is compromised because they do not provide different modes of action
Solution Approach 1:
The invention assigns specific functional qualities to each toxin: Cry1Ac targets one set of insect midgut receptors while Cry1F targets a different set of receptors. This local differentiation in receptor specificity ensures that each toxin provides a distinct mode of action, preventing cross-resistance and enhancing durability while maintaining manageable genetic complexity through specialized expression cassettes.
Data Source
AI summary
The invention provides a transgenic Glycine max event MON87751, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event MON87751. The invention also provides polynucleotides specific for event MON87751, plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides for event MON87751. The invention also provides methods related to event MON87751.