MON95275 Transgenic Corn Event With Multi-Mode Rootworm Control

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Solution Overview

Problem

Existing transgenic corn events have shown resistance to insect infestations, particularly from Coleopteran species like corn rootworm, but there is a need for novel events that confer resistance to insects that have evolved resistance to previously deployed traits, using non-overlapping modes of action.

Innovation Solution

The development of transgenic corn event MON95275, which expresses Cry75Aa1 and Vip4Da2 proteins and a dsRNA targeting DvSnf7, provides resistance to Coleopteran pests through a unique DNA construct optimized for insecticidal efficacy.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing transgenic corn events use previously deployed toxins for insect resistance, then insect resistance is provided, but resistance evolves in targeted insect pests reducing effectiveness

Engineering Contradiction:

Improveinsect resistance effectivenessVSAvoidinsect pest resistance evolution

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent divides the insect resistance function into multiple independent toxin proteins (Cry75Aa1 and Vip4Da2) with distinct modes of action, rather than relying on a single toxin. This segmentation ensures that resistance to one toxin does not confer resistance to the other, maintaining reliable pest control while preventing rapid adaptation by insect pests.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent creates a composite transgenic event combining two different toxin proteins (Cry75Aa1 from Bacillus thuringiensis and Vip4Da2 from Bacillus cereus) with non-overlapping modes of action. This composite approach provides synergistic insect resistance where pests must evolve resistance to multiple different mechanisms simultaneously, significantly delaying resistance development while maintaining effective pest control.

Inventive Principle:

Principle #40Composite materials

2Duration of action of stationary object

If transgenic corn events are deployed commercially for extended periods, then insect resistance is maintained, but resistance to expressed toxins is observed in many geographic regions

Engineering Contradiction:

Improvecommercial deployment durationVSAvoidtoxin effectiveness

Core Design Contradiction:

Duration of action of stationary objectVSReliability

Solution Approach 1:

The patent introduces dynamic adaptability by combining toxins with different modes of action that can address evolving pest resistance patterns. The Vip4Da2 toxin specifically targets rootworm species that have developed resistance to conventional Cry toxins, allowing the transgenic event to remain effective against emerging resistant populations while maintaining long-term commercial deployment viability.

Inventive Principle:

Principle #15Dynamics

Solution Approach 2:

The patent changes the biochemical parameters of the toxin mechanism by introducing Vip4Da2, which operates through a different mode of action than conventional Cry toxins. This parameter change in toxin mechanism allows the transgenic event to maintain effectiveness against pests that have evolved resistance to previously deployed toxins, extending the durable period of commercial deployment.

Inventive Principle:

Principle #35Parameter changes

3Reliability

If novel transgenic events use non-overlapping modes of action, then resistance to target insects is conferred, but complexity of DNA construct increases

Engineering Contradiction:

Improveresistance to resistant insect racesVSAvoidDNA construct complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent achieves universality by designing a DNA construct that provides broad-spectrum Coleopteran pest resistance through two toxins with different modes of action. This multi-functional approach allows a single transgenic event to effectively control multiple pest species and resistance types, reducing the need for multiple separate transgenic events while managing DNA construct complexity through efficient genetic organization.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

MON95275 effectively controls Coleopteran pests such as Western and Northern Corn Rootworm, offering superior resistance compared to other events, and reduces the need for chemical insecticides, lowering labor and environmental impact.

Implementation Method 1

recombinant DNA molecule present in and/or isolated from corn event MON95275

Methodology Applied

Scientific EffectRecombinant DNA integration:

Implementation Method 2

expresses Cry75Aa1 and Vip4Da2 proteins

Methodology Applied

Scientific EffectTransgene expression:

Implementation Method 3

a dsRNA targeting DvSnf7

Methodology Applied

Scientific EffectRNA interference:

Data Source

PatentUS12454700B2Transgenic corn event MON95275

Publication Date: 2025.10.28 MONSANTO TECHNOLOGY LLC

AI summary

The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.