Monascus purpureus NTU 568 DNA Marker Strain Identification

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Solution Overview

Problem

The identification and DNA molecular marker technology for Monascus purpureus NTU 568 have not been fully established, hindering rapid strain identification without culturing isolated bacteria.

Innovation Solution

A mutant of Monascus purpureus NTU 568 with specific nucleotide sequences and primers for PCR and RAPD processes are provided, enabling strain identification using DNA molecular markers without the need for culturing live bacteria.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If traditional strain identification methods are used, then accurate identification can be achieved, but time-consuming culturing processes are required

Engineering Contradiction:

Improvestrain identification accuracyVSAvoididentification time

Core Design Contradiction:

Measurement precisionVSLoss of time

Solution Approach 1:

The patent extracts specific DNA sequences (ITS region, actin gene, beta-tubulin gene) from the Monascus purpureus NTU 568 strain to create molecular markers. By isolating and sequencing these specific genetic regions, the invention enables direct DNA-based identification without requiring time-consuming culturing processes, thus resolving the contradiction between identification accuracy and time consumption

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent creates DNA copies of the Monascus purpureus NTU 568 strain through molecular cloning and sequencing. By working with replicated DNA sequences rather than live cultures, the identification process can be performed rapidly using PCR and sequencing techniques while maintaining high accuracy through comparison with reference sequences

Inventive Principle:

Principle #26Copying

2Productivity

If DNA molecular marker technology is implemented, then rapid strain identification is enabled, but specific nucleotide sequences and markers must be established first

Engineering Contradiction:

Improveidentification efficiencyVSAvoidmolecular marker system complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The patent divides the Monascus purpureus NTU 568 genome into specific functional segments for marker development, including the ITS region (nuclear ribosomal DNA), actin gene, and beta-tubulin gene. By focusing on these discrete, functionally important segments rather than attempting to analyze the entire genome, the invention achieves rapid identification through targeted sequencing while managing the complexity of molecular marker system development

Inventive Principle:

Principle #1Segmentation

3Measurement precision

If complete strain identification is performed, then accurate characterization is achieved, but extensive culturing and analysis of isolated bacteria are required

Engineering Contradiction:

Improvestrain characterization accuracyVSAvoidoperational simplicity

Core Design Contradiction:

Measurement precisionVSEase of operation

Solution Approach 1:

The patent replaces mechanical/biological culturing operations with molecular biology techniques. Instead of requiring extensive culturing, isolation, and morphological analysis of live bacteria, the invention uses DNA extraction, PCR amplification, and sequencing to achieve complete strain characterization. This substitution maintains high characterization accuracy while dramatically simplifying the operational procedures

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Facilitates rapid and accurate identification of Monascus purpureus NTU 568, enhancing the efficiency of strain verification and production monitoring.

Implementation Method 1

the nucleotide sequence of the Monascus purpureus NTU 568 can be formed by treating the RAPD (Random Amplification of Polymorphic DNA) and the PCR (Polymerase Chain Reaction) process to a plurality of specific primers

Methodology Applied

Scientific EffectPCR (Polymerase Chain Reaction):

Implementation Method 2

Methodology Applied

Scientific EffectRAPD (Random Amplification of Polymorphic DNA):

Data Source

PatentUS9157057B2Monascus purpureus mutant, nucleotide sequence for monascus purpureus mutant and primers for nucleotide sequence of monascus purpureus mutant

Publication Date: 2015.10.13 HSIAO HSIA CHING

AI summary

The present invention relates to a mutant of Monascus purpureus NTU 568, a nucleotide sequence for Monascus purpureus NTU 568 and primers for nucleotide sequence of Monascus purpureus NTU 568, wherein the Monascus purpureus NTU 568 having the nucleotide sequence of SEQ ID NO 1, SEQ ID NO 2 or SEQ ID NO 3 is deposited with Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ, Inhoffenstr. 7B, D-38124 Braunschweig, Germany) on Nov. 18, 2013, with the accession number of DSM 28072. Moreover, the nucleotide sequence for NTU 568 and the primers for the nucleotide sequence are proposed in order to facilitate the person skilled in Monascus purpureus filed capable of carrying out the strain (mutant) identification of the NTU 568 according to the present invention. Moreover, the person skilled in Monascus purpureus filed can also rapidly complete the strain (mutant) identification of the NUT 568 by using DNA molecular marker technology, without culturing any isolated Monascus purpureus strain or live Monascus purpureus bacteria.