Monocot Embryo Extraction via Proximal End Inversion
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Solution Overview
Problem
Current methods for extracting monocot embryos from monocot seeds often damage the embryos due to the application of significant forces required, and existing systems are not suitable for monocot embryos, leading to stress and incomplete extraction.
Innovation Solution
A system and method that involves removing monocot seeds from their biological carrier, orienting them in a liquid bath, and applying force through the proximal end to extract the embryo without damaging it, allowing for undamaged extraction and separation from other seed materials.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If significant forces are applied to extract embryos through the distal end (crown) of seeds, then extraction is achieved, but the embryos are damaged and stressed
Solution Approach 1:
The patent inverts the conventional extraction approach by extracting embryos through the proximal end of the seed rather than the distal end (crown). This reversal allows the embryo to be pulled through the micropyle region, which requires significantly less force and causes minimal damage to the embryo, thereby resolving the contradiction between extraction efficiency and embryo integrity
Solution Approach 2:
The patent applies preliminary action by pre-softening the seed coat through hydrolysis treatment before extraction. This preliminary step weakens the seed structure, making it easier to access and extract the embryo through the proximal end without requiring excessive force that would damage the embryo
2Productivity
If current extraction systems are used, then extraction is performed, but portions of the seed other than the embryo are extracted and further sieving is required
Solution Approach 1:
The patent applies the extraction principle by specifically removing only the embryo portion from the seed through the proximal end, leaving the rest of the seed intact. This selective extraction eliminates the need for subsequent sieving steps to separate embryo from other seed materials, thereby reducing process complexity while maintaining throughput
Solution Approach 2:
By inverting the extraction direction to use the proximal end, the patent achieves cleaner embryo extraction without附带 seed materials. The proximal end extraction path naturally separates the embryo from the bulk seed material, eliminating the need for complex sieving equipment and processes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables the extraction of undamaged monocot embryos with reduced stress and without the need for sieving, maintaining the viability and vigor of the embryos for further use, such as plant regeneration.
Implementation Method 1
A monocot seed can be obtained and then placed in a liquid bath to achieve a desired orientation
Implementation Method 2
placed in a liquid bath to achieve a desired orientation
Implementation Method 3
a force applied to the monocot seed to extract the monocot embryo
Data Source
Figure 1~2A
Figure 2B
Figure 3A
AI summary
Methods and systems are provided which permit extraction of a monocot embryo (e.g., a corn embryo) of a monocot seed (e.g., a corn kernel) without damage to the monocot embryo. Methods disclosed herein provide embryos for uses in plant breeding and research procedures.