Monocyte Gene Expression Detection in Peripheral Blood
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Solution Overview
Problem
Current methods for detecting gene expression in single cell subpopulations from peripheral blood samples are either expensive and limited to research settings or require cumbersome cell isolation steps, making them unsuitable for clinical applications.
Innovation Solution
A method for directly measuring the transcript abundance of monocyte-specific genes in cell-mixture samples, such as peripheral blood, without isolating monocytes, using a kit with reagents for quantifying gene transcript abundances.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If single cell RNA-sequencing is used to obtain gene expression information of single cells, then measurement precision is improved, but cost increases significantly making it unsuitable for clinical applications
Solution Approach 1:
The patent extracts only the necessary information (monocyte-specific gene expression) from the complex whole blood sample using targeted primers and probes, rather than sequencing all RNA in each cell. This selective extraction approach maintains detection accuracy for the specific cell type while dramatically reducing costs and enabling clinical application.
Solution Approach 2:
The patent uses disposable, pre-designed primer and probe sets that can be easily replaced, eliminating the need for expensive, complex sequencing equipment. The reagents are optimized for single-use or limited-use applications, reducing overall cost while maintaining measurement precision for monocyte gene expression.
2Quantity of substance
If conventional methods are used to detect gene expression from cell-mixture samples, then cost is reduced, but cell isolation steps are required making the process cumbersome
Solution Approach 1:
The patent uses fluorescently labeled primers and probes as intermediaries that specifically bind to monocyte mRNA in the complex blood sample. These intermediaries enable direct detection of monocyte gene expression without requiring physical separation or isolation of monocytes from other blood cells, simplifying the operation while maintaining cost-effectiveness.
Solution Approach 2:
The patent replaces mechanical cell isolation procedures (such as centrifugation, filtration, or flow cytometry separation) with a molecular biology approach using sequence-specific primers and probes. This substitution eliminates the need for complex mechanical separation steps while maintaining the ability to detect gene expression specifically in monocytes.
3Ease of operation
If existing clinical tests are used for fever differentiation, then ease of operation is maintained, but measurement precision and specificity are insufficient requiring doctor experience
Solution Approach 1:
The patent changes the detection parameter from general clinical symptoms and serum proteins to specific monocyte gene expression levels. By measuring the abundance of monocyte-specific transcripts (such as those involved in innate immunity and inflammation), the test provides objective, quantifiable data that improves diagnostic specificity while maintaining ease of operation through automated fluorescence measurement.
Data Source
AI summary
The present application provides a peripheral blood sample analysis method and a use of a reagent component for measuring the abundance of a gene transcript in the preparation of a kit for the peripheral blood sample analysis method. The present application also provides a kit comprising the reagent component for quantifying the abundance of the gene transcript and a use of the reagent component for quantifying the abundance of the gene transcript in the preparation of a kit or drug for differentiating and triage of patients having abnormal body temperature.