Monodisperse Polyol Linkers for Uniform Analyte Detection
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Solution Overview
Problem
Existing polymer-based linkers, such as PEG, exhibit polydispersity and solubility issues, complicating the analysis and uniformity of conjugates used in analyte detection assays.
Innovation Solution
Development of monodisperse linker molecules with polyols that enhance hydrophilicity and stability, forming complexes with analyte-specific binding agents and label compounds to improve signal-to-noise ratio in analyte detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If PEG-based linkers are used for conjugation, then hydrophilicity is improved, but polydispersity increases and solubility becomes insufficient
Solution Approach 1:
The patent changes the chemical structure parameters of the linker by replacing PEG-based polymers with defined polyol-based small molecules. This structural parameter change eliminates polydispersity while maintaining hydrophilicity through multiple hydroxyl groups, directly resolving the contradiction between hydrophilicity and manufacturing precision
Solution Approach 2:
The invention uses homogenous polyol-based linkers with uniform molecular weight and structure instead of polydisperse PEG polymers. This homogeneity ensures consistent conjugate properties and eliminates the solubility issues associated with mixed molecular weights, resolving the contradiction between hydrophilicity and manufacturing precision
2Object-affected harmful factors
If PEG-based linkers are used for conjugation, then hydrophilicity is improved, but conjugate solubility becomes insufficient
Solution Approach 1:
The patent changes the molecular weight parameter to a single uniform value rather than a distribution, and modifies the chemical structure to include multiple hydroxyl groups. This parameter change enhances water solubility through increased hydrogen bonding capability while eliminating the solubility problems caused by polydispersity, resolving the contradiction between hydrophilicity and conjugate solubility
3Object-affected harmful factors
If polysaccharides are used as linkers, then hydrophilicity is improved, but structural uniformity decreases due to complexity
Solution Approach 1:
The patent extracts the essential hydrophilic functionality (multiple hydroxyl groups) from complex polysaccharide structures and implements it in simplified polyol-based linkers. This extraction maintains the hydrophilicity benefit while eliminating the structural complexity and variability inherent in polysaccharides, resolving the contradiction between hydrophilicity and structural uniformity
4Ease of manufacture
If polydisperse linkers are used, then ease of manufacture is improved, but analysis complexity increases
Solution Approach 1:
The patent changes the molecular weight parameter from a distribution to a single uniform value, and changes the chemical structure to a well-defined polyol-based small molecule. This parameter change simplifies analytical characterization while maintaining ease of manufacture through available synthetic methods, resolving the contradiction between ease of manufacture and analysis complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of monodisperse polyol linkers results in stable complexes with improved signal-to-noise ratio and simplified product purification, enhancing the reproducibility and uniformity of analyte detection assays.
Implementation Method 1
the label compound is capable of producing a detectable signal, preferably a chemiluminescence based signal, more preferably an electrochemiluminescence based signal
Implementation Method 2
the label compound is capable of producing a detectable signal, preferably a chemiluminescence based signal, more preferably an electrochemiluminescence based signal
Implementation Method 3
certain linker molecules with polyols not only offer superior hydrophilicity over PEG-containing derivatives
Data Source
AI summary
The present invention relates to a method for determining at least one analyte of interest and the use thereof. The present invention further relates to a kit, a complex, a method to synthesize a complex, a monomer and the use thereof for detecting the analyte of interest in the sample.