Movable Excitation Light Source for Fluorescence Imaging
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Solution Overview
Problem
Conventional imaging apparatuses face issues with varying light intensity and illumination distribution due to changes in object position, leading to inconsistent image quality and the need for multiple shading correction settings for different object heights.
Innovation Solution
The imaging method and apparatus involve an object support section that can be moved within the casing to adjust the imaging distance between the imaging section and the object, with an excitation light source secured to the support section to maintain consistent light distribution, allowing for efficient image correction using pre-set conditions based on the illumination distribution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If the light source position is fixed and objects of different sizes are imaged, then the imaging apparatus structure is simple, but the light intensity and illumination distribution vary leading to poor image quality
Solution Approach 1:
The light source is made movable relative to the object support section, allowing dynamic adjustment of the light source position to match different object heights. This resolves the contradiction by enabling the system to adapt its configuration rather than being fixed, thereby maintaining consistent illumination and excitation conditions across varying object sizes without fundamentally complicating the apparatus structure.
Solution Approach 2:
The system changes the positional parameter of the light source relative to the object, specifically adjusting the distance and angle of illumination based on object height. This parameter adjustment ensures that light intensity and illumination distribution remain consistent despite variations in object size, thereby improving image quality while maintaining structural simplicity.
2Adaptability or versatility
If the object position is adjusted to accommodate different object sizes, then the imaging apparatus can handle various objects, but the light intensity and illumination distribution fluctuate causing image quality degradation
Solution Approach 1:
Instead of only moving the object, the system dynamically adjusts the light source position in coordination with object placement. This ensures that regardless of object size or position, the illumination conditions remain consistent, thereby maintaining image quality while preserving adaptability to different objects.
Solution Approach 2:
The system changes the illumination parameters (light source position, angle, and distance) based on the object characteristics. This parameter adjustment compensates for variations in object position and size, ensuring consistent light intensity and illumination distribution across all imaging conditions.
3Measurement precision
If multiple correction tables are created for different object heights, then image correction accuracy improves, but the system complexity and operation time increase
Solution Approach 1:
A single correction table is created in advance based on the standardized illumination conditions achieved by the movable light source. Since the light source can be positioned to provide consistent illumination regardless of object height, one correction table suffices for all object sizes, eliminating the need for multiple correction tables and reducing system complexity.
Solution Approach 2:
The correction table becomes universal, applicable to all object heights and sizes. The movable light source ensures that illumination conditions match the conditions under which the correction table was created, allowing the same correction parameters to be used across all imaging scenarios, thereby simplifying the correction system while maintaining accuracy.
4Measurement precision
If multiple correction tables are created for different object heights, then image correction accuracy improves, but the operation time and efficiency decrease
Solution Approach 1:
The correction table is created once in advance and stored for repeated use. The movable light source ensures that all subsequent imaging operations occur under conditions matching the correction table, eliminating the need to create or switch between multiple correction tables during operation, thereby maintaining accuracy while improving efficiency.
Solution Approach 2:
The system maintains continuous operation with a single correction table applicable to all objects. The movable light source enables consistent illumination conditions across all imaging tasks, allowing the correction process to continue efficiently without interruption for creating or switching correction tables, thereby maintaining both accuracy and productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances image quality by maintaining consistent light intensity and distribution, reducing the need for multiple correction settings and simplifying the imaging process by allowing for automatic adjustment of object height without altering imaging conditions.
Implementation Method 1
light having been produced by a light source located within the casing is irradiated to the object... the fluoro chrome may then be excited with excitation light to produce fluorescence
Implementation Method 2
the fluorescence produced by the labeling substance, such as the fluorescent substance or the fluoro chrome, with which the organism-originating substance has been labeled, is photoelectrically detected
Data Source
AI summary
An imaging section is secured to a casing, and an object is supported on an object support section, which is located within the casing. The object support section is moved within the casing in order for an imaging distance between the imaging section and the object, which has been supported on the object support section, to be altered. Excitation light is irradiated to the object, which has been supported on the object support section, with an excitation light source, which is secured to the object support section. A distribution of illuminances of the excitation light on the object is thus prevented from fluctuating to a fluctuation of the imaging distance.