Functionalized Polystyrene Microspheres for Room-Temperature mRNA Storage
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Solution Overview
Problem
The structural instability of mRNA during storage and transportation leads to degradation, necessitating ultra-low temperature preservation, which is costly and poses safety risks, while external protective agents compromise biosafety.
Innovation Solution
A functionalized polystyrene microsphere with specific functional groups is used to stabilize mRNA at room temperature, forming a three-dimensional structure that maintains RNA integrity through interaction and subsequent separation, allowing for stable storage without cryopreservation or harmful additives.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If ultra-low temperature preservation is used to store mRNA, then the degradation rate is minimized and storage stability is improved, but energy consumption increases, transportation costs increase, and safety issues arise
Solution Approach 1:
The patent changes the storage temperature parameter from ultra-low temperature ( -80°C or liquid nitrogen) to room temperature (25°C) by introducing a stabilizer system. This parameter change eliminates the need for expensive ultra-low temperature refrigeration while maintaining mRNA stability through chemical stabilization mechanisms
Solution Approach 2:
The patent introduces an intermediary stabilizer system consisting of a stabilizer protein and stabilizer RNA that mediate between the mRNA and the storage environment. This intermediary protects the mRNA from degradation at room temperature without requiring extreme temperature conditions
2Stability of the object's composition
If ultra-low temperature preservation is used to store mRNA, then the degradation rate is minimized and storage stability is improved, but transportation costs and safety issues increase
Solution Approach 1:
The patent changes the storage temperature parameter from ultra-low temperature to room temperature, eliminating the safety risks associated with liquid nitrogen and ultra-low temperature refrigeration while maintaining mRNA stability through chemical stabilization
3Stability of the object's composition
If external protective agents are added to stabilize mRNA, then storage stability is improved, but biosafety is compromised
Solution Approach 1:
The patent introduces an intermediary stabilizer system consisting of a stabilizer protein and stabilizer RNA that mediate between the mRNA and the storage environment. This intermediary protects the mRNA from degradation at room temperature without requiring extreme temperature conditions
Solution Approach 2:
The stabilizer protein and stabilizer RNA form a self-contained stabilizing system that actively protects the mRNA without requiring external harmful agents. The system self-assembles and functions to maintain mRNA structure and prevent degradation
4Ease of manufacture
If conventional storage methods are used for mRNA, then existing protocols are simple, but long-term storage stability and biosafety are compromised
Solution Approach 1:
The patent introduces an intermediary stabilizer system consisting of a stabilizer protein and stabilizer RNA that mediate between the mRNA and the storage environment. This intermediary protects the mRNA from degradation at room temperature without requiring extreme temperature conditions
Solution Approach 2:
The stabilizer protein and stabilizer RNA form a self-contained stabilizing system that actively protects the mRNA without requiring external harmful agents. The system self-assembles and functions to maintain mRNA structure and prevent degradation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables long-term stable storage and transportation of mRNA at room temperature, reducing costs and enhancing biosafety with effective stabilization and easy separation.
Implementation Method 1
The functionalized polystyrene microsphere has good adsorption capacity for RNA. The functional groups on the microsphere can interact with the groups in the RNA
Implementation Method 2
The functional groups on the microsphere can interact with the groups in the RNA, so that the RNA molecules rotate, twist, entangle and fold to form a specific three-dimensional structure
Implementation Method 3
The addition of water can destroy the binding between the functionalized polystyrene microsphere and the RNA, so as to achieve complete separation of the RNA from the stabilizer
Data Source
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AI summary
An RNA room-temperature storage stabilizer, an RNA product, and a preparation method and use thereof are disclosed, belonging to the field of biotechnology. The RNA room-temperature storage stabilizer provided by the present disclosure includes a functionalized polystyrene microsphere. The RNA room-temperature storage stabilizer has good adsorption capacity for RNA. The functional groups on the RNA room-temperature storage stabilizer can interact with the groups in the RNA, which plays a role in stabilizing the structure of the RNA, thereby realizing long-term stable storage of the RNA at room temperature without the need of introducing an ultra-low temperature device and other protective reagents; and the addition of water can destroy the binding between the functionalized polystyrene microsphere and the RNA, so as to achieve complete separation of the RNA from the stabilizer. It has the advantages of low cost, simple operation and high biosafety.