Bridging ELISA Immunoassay for MS Diagnosis
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Solution Overview
Problem
Current diagnostic methods for Multiple Sclerosis (MS) are inadequate for early detection and monitoring, as they are not specific and require repetitive clinical and MRI investigations, which are time-consuming and costly.
Innovation Solution
A bridging ELISA immunoassay using MSBI1.176 or MSBI2.176 Rep protein or fragments thereof as antigens to detect antibodies in a subject's sample, providing a specific and sensitive method for diagnosing and monitoring MS.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If current diagnostic methods for Multiple Sclerosis are used, then diagnosis can be performed, but the methods are not specific and require repetitive clinical and MRI investigations which are time-consuming and costly
Solution Approach 1:
The invention segments the diagnostic process into a specific immunological assay targeting anti-MSBI Rep antibodies, separating it from general clinical evaluations and MRI investigations. This specific immunological test can be performed independently to provide definitive diagnostic information, eliminating the need for repetitive general investigations and significantly reducing diagnostic time while maintaining high specificity.
2Measurement precision
If current diagnostic methods for Multiple Sclerosis are used, then diagnosis can be performed, but the methods are not specific and require repetitive clinical and MRI investigations which are costly
Solution Approach 1:
The diagnostic approach is segmented into a specific immunological assay that targets anti-MSBI Rep antibodies, allowing for a definitive diagnosis to be obtained through a single specialized test rather than multiple expensive clinical and MRI investigations. This segmentation eliminates redundant testing and significantly reduces the overall diagnostic cost while maintaining high diagnostic specificity.
3Measurement precision
If a specific immunological assay using MSBI Rep protein is used, then diagnostic specificity is improved, but the method requires development of new assay systems
Solution Approach 1:
The invention uses MSBI Rep protein as an intermediary antigen in the immunological assay. This specific protein antigen serves as a mediator that enables highly specific detection of anti-MSBI Rep antibodies in patient samples. The use of this well-defined intermediary antigen simplifies the assay system while achieving high diagnostic specificity, as the protein-based antigen can be readily produced and standardized.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively distinguishes between MS patients and healthy controls, enabling early diagnosis and monitoring of disease progression and therapeutic success, thus improving patient outcomes.
Implementation Method 1
A bridging ELISA immunoassay using MSBI1.176 or MSBI2.176 Rep protein or fragments thereof as antigens to detect antibodies in a subject's sample
Data Source
AI summary
The present invention provides a method for diagnosing and monitoring MS on the basis of an immunoassay using MSBI1.176 or MSBI2.176 Rep protein or fragments thereof as an antigen for binding antibodies against MSBI1.176 or MSBI2.176 Rep protein from a subject's sample.