Mt12a Subtilase Regulatory Elements for Symbiotic Infection
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Solution Overview
Problem
Farmers face challenges due to nitrogen-deficient or phosphate-deficient soils, leading to low yields or plant death, and there is a lack of understanding of the genetic basis and molecular mechanisms for symbiotic nitrogen fixation.
Innovation Solution
Development of novel gene regulatory elements and DNA constructs that include a subtilase protein or fragment thereof, specifically designed to enhance symbiotic nitrogen fixation in plants by promoting symbiotic infection with rhizobia bacteria or arbuscular mycorrhiza fungi.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If symbiotic bacteria are used to improve plant biomass under low-nitrogen conditions, then plant growth is improved, but the genetic basis and molecular mechanisms for symbiotic nitrogen fixation are not fully understood
Solution Approach 1:
The patent performs preliminary identification and characterization of the Mt12a subtilase gene and its regulatory elements before applying them to improve symbiotic nitrogen fixation. By预先 identifying the gene's function and expression patterns through mutational analysis and reporter assays, the patent establishes a molecular basis for subsequent genetic engineering applications.
Solution Approach 2:
The Mt12a subtilase gene acts as an intermediary molecule that mediates between the plant host and rhizobial bacteria during symbiotic infection. The gene product facilitates infection thread formation and bacterial colonization, bridging the interaction gap between host and symbiont at the molecular level.
2Productivity
If intracellular colonization by symbionts is promoted to increase growth under low-nitrogen conditions, then agronomic performance is improved, but the molecular mechanisms remain unclear
Solution Approach 1:
The patent identifies that Mt12a expression is specifically localized to infection threads and nodule primordia during symbiotic development. This spatially restricted expression pattern indicates that the subtilase functions locally at critical infection sites rather than systemically throughout the plant, providing insight into the localized molecular mechanisms of symbiosis.
Solution Approach 2:
The patent performs preliminary functional analysis of Mt12a through complementation of mutant phenotypes and overexpression studies, establishing the gene's role in infection thread formation before applying this knowledge to improve agronomic performance through genetic engineering.
3Productivity
If regulatory elements are developed to enhance symbiotic nitrogen fixation, then nitrogen fixation is improved, but device complexity increases
Solution Approach 1:
The patent extracts the specific regulatory elements (promoter regions) from the Mt12a gene that control its symbiosis-specific expression. By isolating and characterizing these regulatory sequences independently, the patent enables their use as modular components in genetic engineering without requiring manipulation of the entire gene structure.
Solution Approach 2:
The patent segments the Mt12a regulatory system into distinct functional components: the subtilase coding region, the promoter region, and tissue-specific regulatory elements. This segmentation allows independent manipulation and combination of these elements to create simplified genetic constructs for enhancing nitrogen fixation.
Data Source
AI summary
The present disclosure provides novel DNA molecules and constructs encoding proteins for promoting or increasing symbiotic infection in plants. The disclosure also provides DNA molecules useful for expressing proteins in an infection-specific expression pattern. The instant disclosure also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the disclosed DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.