MTU–Nitrogen Enhancer Combinations for Nitrogen-Efficient Plant Growth

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Solution Overview

Problem

Current agricultural practices face challenges in maximizing nitrogen utilization by plants while minimizing environmental leakage and enhancing plant growth, stress resistance, and yield with reduced agrochemical use.

Innovation Solution

A combination of 1-(2-methoxyethyl)-3-(1,2,3-thiadiazol-5yl)urea (MTU) with selected nitrogen utilization enhancers such as phosphite, glutamine, or microorganisms like Azotobacter vinelandii, applied synergistically to enhance plant growth and yield.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If nitrogen fertilizer is extensively applied to achieve rapid plant growth and yield maximization, then plant growth and yield are improved, but production costs increase and environmental pollution occurs

Engineering Contradiction:
Improveplant growth and yieldVSAvoidnitrogen leakage to environment
Core Design Contradiction:
ProductivityVSLoss of substance

Solution Approach 1:

The patent combines MTU (a plant growth regulator) with nitrogen utilization enhancers (such as amino acids, nucleotides, or microorganisms) into a single composition. This merging allows the composition to both stimulate plant growth through MTU and improve nitrogen uptake efficiency through the enhancers, thereby reducing the need for excessive nitrogen fertilizer application while maintaining high productivity and minimizing environmental pollution.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The invention creates a composite formulation integrating multiple functional components: MTU as the active growth-regulating agent, nitrogen utilization enhancers (such as pyroglutamic acid, proline, γ-PGA, or nitrogen-fixing microorganisms), and auxiliary substances. This composite material works synergistically to enhance both growth rate and nitrogen use efficiency, allowing reduced nitrogen fertilizer rates while achieving target yields and reducing nitrogen loss to the environment.

Inventive Principle:
Principle #40Composite materials

2Loss of substance

If nitrogen fertilizer application is reduced to minimize environmental pollution and production costs, then environmental impact and costs decrease, but plant growth speed and yield may be compromised

Engineering Contradiction:
Improvenitrogen leakage to environmentVSAvoidplant growth speed and yield
Core Design Contradiction:
Loss of substanceVSProductivity

Solution Approach 1:

The patent merges MTU's growth-promoting action with nitrogen utilization enhancers' ability to improve nitrogen uptake and metabolism. This combination compensates for reduced nitrogen fertilizer rates by enhancing the plant's efficiency in utilizing available nitrogen, thereby maintaining growth speed and yield while reducing nitrogen leakage to the environment.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The invention changes the physiological parameters of nitrogen metabolism in plants through the action of nitrogen utilization enhancers. These enhancers modify enzyme activities, transport mechanisms, and metabolic pathways related to nitrogen assimilation, thereby improving nitrogen use efficiency and maintaining productivity even when external nitrogen supply is reduced.

Inventive Principle:
Principle #35Parameter changes

3Ease of operation

If conventional single-substance treatments are used to simplify application, then ease of operation is improved, but the synergistic effect on nitrogen utilization and growth is limited

Engineering Contradiction:
Improveapplication simplicityVSAvoidnitrogen utilization efficiency
Core Design Contradiction:
Ease of operationVSProductivity

Solution Approach 1:

The patent combines multiple active ingredients (MTU and nitrogen utilization enhancers) into a single integrated composition that can be applied in one treatment. This merging maintains ease of operation comparable to single-substance applications while delivering synergistic effects that significantly enhance nitrogen utilization efficiency and plant growth beyond what either substance could achieve alone.

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The combination demonstrates enhanced plant growth, stress resistance, improved quality traits, and increased yield, with a synergistic effect beyond the sum of individual components.

Implementation Method 1

The nitrogen absorption by plants is limited by the capacity of the plant nitrogen-metabolism cycle

Methodology Applied
Scientific EffectAbsorption: Absorption (physical)

Implementation Method 2

PCA can be converted to glutamate (Mazelis M, Pratt HM. 1976. In vivo conversion of 5-oxoproline to glutamate by higher plants. Plant Physiology 57: 85-87)

Methodology Applied
Scientific EffectEnzymatic conversion: Enzyme

Implementation Method 3

Proline can also be converted to glutamine in plants thus improving nitrogen utilization

Methodology Applied
Scientific EffectMetabolic conversion: Chemical Bonding

Data Source

PatentEP4463002B1Combination for improving plant growth and/or yield, formulation and method for increasing plant growth, stress resistance, and yields, and use thereof
Publication Date: 2025.08.20 INTRACROP LTD
  • EP4463002B1 patent drawingFigure 1A~1B
  • EP4463002B1 patent drawing
  • EP4463002B1 patent drawing

AI summary

The present invention relates to a combination for improving plant growth and/or yield comprising 1-(2-methoxyethyl)-3-(1,2,3-thiadiazol-5yl)urea and at least one nitrogen assimilation enhancer, selected from the group consisting of phosphite, glutamine, glutamate, glycine, glycine betaine, 2-oxoglutarate, glutamic acid, aspartic acid, aspartate, proline, pyroglutamic acid, γ-aminobutyric acid, poly-γ-(glutamic acid), poly(aspartic acid), poly(glutamic acid), diethyl aminoethyl hexanoate, N-acetyl thiazolidine 4-carboxylic acid, thiazolidine 4-carboxylic acid, microorganisms selected from the group comprising Azotobacter vinelandii, Azospirillum brasilense, Pseudomonas fluorescens, Bacillus polymyxa, Bacillus megaterium, Azorhizobium caulinodens, Azoarcus indigens, and Bacillus sp., phosphobacteria, Streptomyces spp. The invention further relates to formulations comprising such combination and to methods of increasing plant growth, stress resistance, and yields.