Multi-amine Linker PNA Probes for Enhanced Immobilization
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Solution Overview
Problem
Existing methods for immobilizing peptide nucleic acid (PNA) probes on solid surfaces are inefficient, leading to challenges in detecting multiple nucleotide sequences simultaneously with high sensitivity and specificity.
Innovation Solution
Conjugating PNA probes with multi-amine linkers to enhance immobilization efficiency on functionalized surfaces, improving the intensity and sensitivity of detection signals by increasing the number of amine functional groups at the probe terminals.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional single-amine PNA probes are used for immobilization, then the device complexity is low, but the immobilization efficiency and detection sensitivity are insufficient
Solution Approach 1:
The patent changes the chemical parameter of the PNA probe by introducing multi-amine linkers with different numbers of amine groups (di-amine, tri-amine, tetra-amine). This modification increases the immobilization efficiency and detection sensitivity without fundamentally changing the probe's core function, thereby resolving the contradiction between detection sensitivity and structural complexity
Solution Approach 2:
The patent creates composite structures by combining PNA probes with multi-amine linker molecules. These composite probes integrate the sequence-specific recognition capability of PNA with the enhanced immobilization properties of multi-amine groups, achieving both high detection sensitivity and controlled complexity
2Productivity
If PNA probes are immobilized on solid surface for multiplex detection, then multiple sequences can be detected simultaneously, but the immobilization efficiency is insufficient leading to weak detection signals
Solution Approach 1:
The patent modifies the chemical parameters of PNA probes by conjugating multi-amine linkers, which significantly enhances immobilization efficiency on solid surfaces. This results in stronger detection signals while maintaining multiplex detection capability, resolving the contradiction between productivity and reliability
Solution Approach 2:
The patent uses multi-amine linkers as intermediary structures that can be efficiently copied and immobilized on solid surfaces. The multiple amine groups provide multiple attachment points, ensuring high immobilization efficiency and strong signal generation across multiple probe locations for multiplex detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of PNA probes with multi-amine linkers significantly enhances the immobilization efficiency and detection signal intensity, enabling more sensitive and specific gene diagnosis and nucleotide sequence detection.
Implementation Method 1
Conjugating PNA probes with multi-amine linkers to enhance immobilization efficiency on functionalized surfaces, improving the intensity and sensitivity of detection signals by increasing the number of amine functional groups at the probe terminals
Data Source
AI summary
The present invention relates to a peptide nucleic acid (PNA) conjugated with multi-amine linkers, and a method to prepare the same and utilization thereof. More specifically, the method is characterized by conjugating monomers having multi-amine functionality sequentially at a PNA terminal, and effectively immobilizing the PNA conjugated with multi-amine linkers on a solid surface. A PNA array prepared using the PNA conjugated with multi-amine linkers exhibits improved sensitivity and specificity of signals for detecting target nucleic acids as compared to a PNA array using PNA probes having only one amine group. The PNA conjugated with multi-amine linkers can be utilized in nucleic acid detecting devices or kits for gene diagnosis such as PNA microarrays, PNA chips, PNA field-effect transistors and impedance detectors.