Multi-Compartment Wastewater Toxicity Testing With Integrated Aeration

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Solution Overview

Problem

Existing OECD 209 toxicity tests for wastewater treatment require significant space, equipment, and human resources, and take too long to complete, limiting their deployment in industries.

Innovation Solution

A compact, multi-compartment device with integrated stirring, aeration, and oxygen measurement modules that allows simultaneous testing of multiple samples, reducing space and time requirements.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If traditional OECD 209 test method is used with large volumes and single-bottle setup, then measurement precision and reliability are maintained, but device complexity and space requirements increase significantly

Engineering Contradiction:
Improvetoxicity evaluation accuracyVSAvoidequipment and space requirements
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The invention divides the testing system into multiple independent compartments (at least two compartments) within a single device, allowing parallel testing of multiple samples. Each compartment is equipped with its own stirring module, aeration module, and oxygen measurement module, enabling simultaneous independent measurements that reduce overall device complexity while maintaining precision through replication.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention combines multiple testing functions (stirring, aeration, oxygen measurement) into an integrated multi-compartment device. By merging these functions into a unified system with shared control mechanisms while maintaining independent measurement capabilities, the device reduces space requirements and operational complexity compared to traditional separate equipment setups.

Inventive Principle:
Principle #5Merging (Combining)

2Measurement precision

If traditional single-bottle testing with sequential setup is used, then measurement precision is maintained, but productivity and time efficiency deteriorate

Engineering Contradiction:
Improvetoxicity evaluation accuracyVSAvoidtesting throughput
Core Design Contradiction:
Measurement precisionVSProductivity

Solution Approach 1:

The device segments the testing process into multiple parallel compartments, allowing simultaneous testing of multiple samples. This segmentation enables multiple toxicity evaluations to occur concurrently rather than sequentially, significantly increasing productivity while maintaining the measurement precision of individual tests through standardized compartment design.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention enables continuous testing by allowing multiple samples to be processed simultaneously in different compartments. While one compartment is being measured, other compartments can be prepared or are already in different stages of the testing process, eliminating idle time and maintaining continuous productive action throughout the device operation.

Inventive Principle:
Principle #20Continuity of useful action

3Measurement precision

If traditional method with multiple replicates and controls is used, then measurement precision and reliability are ensured, but loss of time and productivity increase

Engineering Contradiction:
Improvetest result reliabilityVSAvoidoverall test duration
Core Design Contradiction:
Measurement precisionVSLoss of time

Solution Approach 1:

The device segments the required replicates and controls into different compartments, allowing all necessary repetitions (minimum 3 replicates plus controls) to be conducted simultaneously rather than sequentially. This parallel execution maintains statistical reliability through adequate replication while reducing the total time required from hours to a fraction of that duration.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The device allows preliminary preparation of multiple compartments with different samples, replicates, and controls to be completed before the measurement phase begins. All compartments can be set up in advance with their respective activated sludge, samples, and conditions, so that the actual measurement process can proceed simultaneously across all compartments without sequential delays.

Inventive Principle:
Principle #10Preliminary action

4Measurement precision

If traditional OECD 209 protocol with manual preparation is used, then measurement precision is maintained, but ease of operation deteriorates due to high man-time requirements

Engineering Contradiction:
Improvetoxicity evaluation accuracyVSAvoidoperational simplicity
Core Design Contradiction:
Measurement precisionVSEase of operation

Solution Approach 1:

The invention merges multiple manual operations (stirring, aeration, oxygen measurement) into an integrated automated device. The combined system requires minimal manual intervention after initial sample loading, as all functional modules operate automatically and simultaneously, dramatically reducing the approximately 65% man-time requirement of the traditional method while maintaining measurement precision through consistent automated operation.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The device is designed to perform all testing functions autonomously once samples are loaded. The stirring modules, aeration modules, and oxygen measurement modules operate self-regulated and simultaneously without requiring continuous manual adjustment or monitoring, allowing the system to serve itself and eliminating the need for extensive human resources during the testing process.

Inventive Principle:
Principle #25Self-service

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The device significantly reduces space and time needed for testing by 90% and man-time by 93%, while maintaining result accuracy and reproducibility, offering a cost-effective solution for rapid toxicity evaluation.

Implementation Method 1

a stirring module (18) comprising a rotation shaft (20) driven in rotation by a drive means (38)

Methodology Applied
Scientific EffectMechanical stirring: Stirring

Implementation Method 2

an aeration module (30) comprising a compression chamber (32) connected to a plurality of ventilation capillaries (36)

Methodology Applied
Scientific EffectAeration: Aeration

Implementation Method 3

a measurement module (10) comprising a probe (12) in contact with the aqueous medium and a sensor (14)

Methodology Applied
Scientific EffectOxygen sensing:

Implementation Method 4

This is a method of purification by free cultures consisting of bringing the wastewater into contact with a mixture rich in bacteria to degrade the organic matter

Methodology Applied
Scientific EffectAerobic respiration: Aerobic Digestion

Data Source

PatentEP4444453B1Device for carrying out a test to evaluate wastewater
Publication Date: 2025.09.24 NANTES UNIVERSITÉ (33 33)
  • EP4444453B1 patent drawingFigure 1~2
  • EP4444453B1 patent drawingFigure 3~4
  • EP4444453B1 patent drawingFigure 5~6

AI summary

The invention relates to a device (2) that has a reaction support (4) comprising a plurality of compartments, each formed by a chamber (6) comprising at least a set of three functional modules: - a stirrer module (18) comprising a rotating shaft (20) rotated by a drive means using electricity and/or using a fluid supply, the rotating shaft (20) forming a channel (24) that is able to route a fluid and the rotating shaft (20) comprising at least one fluid outlet port (26) for letting fluid into the chamber (6); - an aeration module (30) comprising an aeration capillary (36); and - a measurement module (10) for measuring the oxygen level in the chamber (6).