Multi-Electrode Redox Sensing for Culture State Inference

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Solution Overview

Problem

Existing systems for inferring culture conditions in microorganism cultures can only provide basic information on whether the culture solution is aerobic or anaerobic, lacking data for optimizing culture operations.

Innovation Solution

A system utilizing multiple types of working electrodes and a reference electrode to measure redox potentials, combined with an information storage unit and dynamics inference unit, to collect and analyze multivariate data for machine learning and optimization.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If a single type of working electrode is used for redox potential measurement, then the device structure remains simple, but the measurement precision and information quality for culture condition inference is insufficient

Engineering Contradiction:
Improvemeasurement precisionVSAvoiddevice complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent divides the measurement system into multiple segments by using three different types of working electrodes (platinum, gold, and glassy carbon electrodes) instead of a single electrode. Each electrode type provides distinct redox potential measurements that collectively offer more precise and comprehensive information about culture conditions, thereby resolving the contradiction between measurement precision and device complexity.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the material parameter of the working electrodes by employing three different materials (platinum, gold, and glassy carbon) with distinct electrochemical properties. This parameter change enables the system to capture multiple aspects of redox potential, improving measurement precision while maintaining a relatively simple overall device structure.

Inventive Principle:
Principle #35Parameter changes

2Loss of information

If basic redox potential measurement is used, then the system structure remains simple, but the information quality for culture operation optimization is insufficient

Engineering Contradiction:
Improveinformation qualityVSAvoiddevice complexity
Core Design Contradiction:
Loss of informationVSDevice complexity

Solution Approach 1:

The patent adds another dimension to the measurement system by incorporating multiple working electrode types that provide multidimensional redox potential data. This dimensional expansion enables comprehensive analysis of culture conditions including aerobic/anaerobic states, metabolic phases, and optimization parameters, significantly reducing information loss without requiring complex additional devices.

Inventive Principle:
Principle #17Another dimension (Dimensionality change)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Provides comprehensive data for determining culture operations and optimizing conditions, enabling advanced analysis and optimization of culture solutions.

Implementation Method 1

measure the redox potential between each working electrode and a reference electrode

Methodology Applied
Scientific EffectRedox potential: Redox Reactions

Data Source

PatentUS12540916B2System for inference of measurement target dynamic state using redox potential
Publication Date: 2026.02.03 CHITOSE LAB
  • US12540916B2 patent drawing
  • US12540916B2 patent drawing
  • US12540916B2 patent drawing

AI summary

[Problem] To provide a system for inference of a measurement target dynamic state, the system being capable of providing not only information as to whether a culture solution is aerobic or anaerobic, but also data for determining a culture operation and for optimizing various conditions of the culture solution. [Solution] A system for inference of a measurement target dynamic state, comprising: a reference electrode; a first working electrode; a second working electrode of which at least the material or surface treatment is different from the first working electrode; a third working electrode of which at least the material or surface treatment is different from the first working electrode and the second working electrode; and an information storage unit which receives first potential information relating to the redox potential between the reference electrode and the first working electrode, second potential information relating to the redox potential between the reference electrode and the second working electrode, and third potential information relating to the redox potential between the reference electrode and the third working electrode, and stores information including the first potential information, the second potential information, and the third potential information.