Multi-Hole Membrane Biosensor for High-Sensitivity Detection
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Solution Overview
Problem
Conventional lateral flow assays have limited detection sensitivity and require larger sample volumes, making them inadequate for detecting analytes at higher concentrations and taking longer to measure enzymatic and immunological reactions.
Innovation Solution
A membrane biosensor with a multi-hole film attached to a receptor-immobilized membrane, allowing for vertical sample injection and adjustable sensitivity through hole size, enabling the detection of multiple analytes with a small sample volume in a short time.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional lateral flow assay is used, then the structure is simple and easy to manufacture, but the detection sensitivity is limited to about 1 ng/mL and cannot detect analytes requiring higher sensitivity
Solution Approach 1:
The membrane is divided into multiple regions with different hole sizes (first through fourth hole sizes), creating segmented detection zones. Each region can detect different analytes or provide different sensitivity levels, enabling high sensitivity detection while maintaining a relatively simple overall structure based on the familiar lateral flow format.
Solution Approach 2:
Different regions of the membrane are assigned different hole sizes to optimize local detection characteristics. Smaller holes provide higher sensitivity for low-concentration analytes, while larger holes allow faster flow and higher throughput. This local differentiation enables high sensitivity detection in specific zones without complicating the entire device structure.
2Quantity of substance
If conventional lateral flow assay is used, then the device is simple to operate, but the sample volume required is large and measurement time is prolonged
Solution Approach 1:
The membrane is segmented into multiple regions with different hole sizes, allowing different sample volumes to be applied to different regions simultaneously. This enables the use of smaller total sample volumes while maintaining ease of operation through a straightforward lateral flow application process.
Solution Approach 2:
The invention transitions from a single uniform membrane structure to a multi-dimensional structure with varying hole sizes across different regions. This dimensional variation allows optimized sample utilization - smaller holes concentrate and retain more analyte per unit volume, reducing the total sample volume needed while keeping the operation simple through lateral flow application.
3Productivity
If conventional lateral flow assay is used, then the measurement process is straightforward, but the detection time is prolonged and rapid detection is not achieved
Solution Approach 1:
The membrane is segmented into regions with different hole sizes, allowing parallel processing of multiple analytes at different detection speeds. Regions with larger holes enable faster sample throughput and quicker detection, improving overall productivity without significantly increasing measurement time for individual analytes.
Solution Approach 2:
By introducing dimensional variation through different hole sizes across the membrane surface, the system enables optimized flow dynamics. Larger holes in certain regions accelerate sample flow and reduce detection time, while smaller holes provide higher sensitivity when needed, achieving rapid detection overall while maintaining straightforward operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The membrane biosensor achieves high sensitivity and rapid detection of various analytes using a small sample volume, outperforming conventional methods by allowing for simultaneous measurement of multiple components and reducing analysis time.
Implementation Method 1
antibody is immobilized to a membrane in which a fluid sample moves via capillary force
Implementation Method 2
The absorption pad is composed of a material capable of efficiently absorbing the fluid sample
Implementation Method 3
the antibody capable of being selectively bound to the sample substance and the antibody immobilized to a gold nanoparticle are configured to be bound to the sample substance in a sandwich manner
Data Source
AI summary
The present invention relates to a membrane sensor having a multi-hole film attached thereto and a method for measuring immunological reactions or enzymatic reactions using the same. More specifically, the present invention relates to a membrane sensor in which a multi-hole film is joined to the top of a membrane on which receptors are immobilized, and a method for measuring immunological reactions or enzymatic reactions using the same. The present invention makes it possible to adjust the sensitivity of membrane biosensors by adjusting the hole size in the multi-hole film and so makes it possible to measure analytes with a high degree of sensitivity using just a small amount of sample, and makes it possible to simultaneously measure diverse types of analyte by attaching various types of receptor on the membrane sensor.