Multi-Lactobacillus Composition for BV Microecological Restoration
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Solution Overview
Problem
Current treatments for bacterial vaginosis (BV) using antibiotics are ineffective in maintaining vaginal microecological balance, leading to high recurrence rates and drug resistance, while existing probiotic products do not utilize dominant lactobacilli strains specific to Chinese females, limiting their effectiveness.
Innovation Solution
A multi-lactobacillus composition comprising Lactobacillus johnsonii Ljohn-1, Lactobacillus gasseri Lgass-17, Lactobacillus rhamnosus Lrham-6, and Lactobacillus crispatus Lcris-2, screened and deposited from healthy Chinese females, is formulated to provide synergistic probiotic benefits and restore vaginal microecological balance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If antibiotic therapy is used to treat bacterial vaginosis, then the treatment effect is quick, but the recurrence rate is high and drug resistance occurs
Solution Approach 1:
Instead of using antibiotics to kill pathogenic bacteria, the patent inverts the approach by using probiotics (lactobacilli) to restore beneficial flora, which naturally suppresses pathogenic bacteria through competition for resources and adhesion sites, thereby reducing recurrence and resistance while maintaining effective treatment
Solution Approach 2:
The patent converts the harmful effect of antibiotic overuse (killing beneficial flora along with pathogens) into a benefit by deliberately introducing specific lactobacillus strains that can quickly establish themselves and restore ecological balance, turning the post-antibiotic vulnerability into an opportunity for targeted probiotic colonization
2Ease of manufacture
If single-strain probiotics are used, then the product is simple to manufacture, but the probiotic ability is limited
Solution Approach 1:
The patent combines four different lactobacillus strains (L. crispatus, L. gasseri, L. jensenii, and L. rhamnosus) into a single multi-strain probiotic product, merging their complementary functions to achieve superior probiotic ability while maintaining manufacturing feasibility through a unified formulation approach
Solution Approach 2:
The patent creates a composite probiotic formulation containing multiple lactobacillus strains with different functional characteristics, where each strain contributes unique probiotic mechanisms (H2O2 production, lactic acid production, bacteriocin secretion, adhesion capabilities), resulting in a synergistic composite product with enhanced overall effectiveness
3Reliability
If probiotics are used to restore vaginal microecological balance, then the recurrence rate is reduced, but the treatment effect is slower compared to antibiotics
Solution Approach 1:
The patent employs lactobacillus strains that are pre-adapted and pre-cultured to ensure rapid colonization capability, allowing them to quickly establish themselves in the vaginal environment and begin restoring ecological balance immediately upon administration, thus accelerating the probiotic treatment effect
Solution Approach 2:
The patent optimizes key parameters including the viability concentration (10^8-10^10 CFU/g), moisture content (3-15%), and composition ratios of different strains to maximize colonization efficiency and probiotic activity, thereby enhancing the speed of treatment effect while maintaining low recurrence rates
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The multi-lactobacillus composition enhances probiotic ability, effectively inhibiting pathogenic bacteria and restoring vaginal health, offering a wide range of treatment efficacy for BV with reduced recurrence.
Implementation Method 1
They may not only grow by virtue of glycogen inside the vaginal epithelial cells and produce H2O2, lactic acid and bacteriocin
Implementation Method 2
They may not only grow by virtue of glycogen inside the vaginal epithelial cells and produce H2O2, lactic acid and bacteriocin
Implementation Method 3
They may not only grow by virtue of glycogen inside the vaginal epithelial cells and produce H2O2, lactic acid and bacteriocin, but also may be competitively adhered to the vaginal epithelium to occupy a binding site
Implementation Method 4
They may not only grow by virtue of glycogen inside the vaginal epithelial cells and produce H2O2, lactic acid and bacteriocin
Data Source
AI summary
A multi-lactobacillus composition is a dominant strain separated and selected from the vagina of a healthy woman in China. The multi-lactobacillus composition comprises at least three of the following active components: Lactobacillus johnsonii, Lactobacillus gasseri, Lactobacillus rhamnosus, Lactobacillus crispatus, and Lactobacillus jensenii.