Multi-layer Cell Culture Container with Nested Prisms
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing cell culture containers, such as T-flasks and roller bottles, are inadequate for large-scale cultivation, and existing solutions like accordion-like containers do not provide sufficient internal surface area for adherent mammalian cell growth.
Innovation Solution
A multi-layer cell culture container with a polygonal prism outer body and multiple coaxial inner layer structures, equipped with spacers and valves, providing a large internal surface area and convenient gas/liquid injection without contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If the container size is increased to provide more surface area for cell growth, then the internal surface area increases, but the container volume and footprint increase proportionally, making it unsuitable for large-scale cultivation in limited spaces
Solution Approach 1:
The patent employs nested inner layer structures within the outer body, where multiple concentric polygonal prisms are arranged one inside another. Each inner layer structure provides additional internal surface area for cell attachment while occupying minimal additional volume. The spacers positioned between layers maintain optimal spacing without requiring excessive container volume, thus resolving the contradiction between maximizing surface area and minimizing container volume.
Solution Approach 2:
The patent transitions from a two-dimensional surface expansion approach to a three-dimensional spatial arrangement by creating multiple concentric layers. This dimensional transformation allows the container to provide large internal surface area through vertical and radial stacking of layers rather than horizontal expansion, effectively increasing surface area without proportional increase in container volume.
2Ease of manufacture
If conventional containers like T-flasks and roller bottles are used, then they are readily available and easy to use, but they provide insufficient internal surface area for large-scale cell cultivation
Solution Approach 1:
The patent divides the container into modular components including the outer body, multiple detachable inner layer structures, spacers, and covers. This segmentation allows the container to be manufactured using standard techniques for each component while enabling easy assembly. The modular design maintains ease of manufacture through standardized parts while achieving large internal surface area through the configurable multi-layer structure.
3Area of stationary object
If the container is designed with complex multi-layer structures to increase surface area, then internal surface area increases for large-scale cultivation, but the device complexity increases
Solution Approach 1:
The patent designs the inner layer structures with universal geometric features (concentric polygonal prisms with uniform spacing) that can be replicated and scaled. The spacers serve multiple functions: maintaining layer spacing, providing structural support, and facilitating assembly. This universality reduces design complexity while enabling the multi-layer configuration that provides large internal surface area.
Data Source
AI summary
A cell culture container is provided. The cell culture container has a multilayer structure inside the cell culture vessel. Therefore, the number of cells obtained per occupied volume is greatly improved and efficient mass cell culture can be achieved. Further, a cap for closing the opening has or is connected with a valve capable of injecting arbitrary gas or liquid while preventing contamination of bacteria, etc., so that the gas phase inside any cell culture vessel can be easily replaced.