Simultaneous Multi-Modal Microscopy for Digital Tissue Staining
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Solution Overview
Problem
Existing methods for digitally staining biological tissue probes require precise alignment of image pairs, leading to inefficient processing times and potential artifacts due to morphological differences, and often rely on single imaging modalities which limit image quality and frame rate.
Innovation Solution
The method employs simultaneous multi-modal microscopy to generate digitally stained images, eliminating the need for precise alignment and incorporating multiple imaging modalities to enhance image quality and processing speed, while using artificial intelligence systems trained with unaligned image pairs to predict digitally stained images.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of information
If sequential imaging of multiple modalities is used, then comprehensive tissue information is obtained, but processing time increases and frame rate decreases
Solution Approach 1:
The patent combines multiple imaging modalities (transmission light, reflection light, fluorescence) into a single simultaneous imaging system. The microscope is configured with multiple light sources and detection channels that operate concurrently on the same tissue section, merging the capabilities of separate imaging systems into one unified platform that captures all modalities at the same time point.
Solution Approach 2:
The system enables continuous simultaneous acquisition of multiple imaging modalities without sequential processing delays. By using parallel detection channels and synchronized imaging, the system maintains continuous useful action across all modalities, eliminating the time losses associated with sequential scanning and processing of different imaging types.
2Loss of information
If sequential imaging of multiple modalities is used, then comprehensive tissue information is obtained, but frame rate decreases
Solution Approach 1:
The patent combines multiple imaging modalities (transmission light, reflection light, fluorescence) into a single simultaneous imaging system. The microscope is configured with multiple light sources and detection channels that operate concurrently on the same tissue section, merging the capabilities of separate imaging systems into one unified platform that captures all modalities at the same time point.
Solution Approach 2:
The system enables continuous simultaneous acquisition of multiple imaging modalities without sequential processing delays. By using parallel detection channels and synchronized imaging, the system maintains continuous useful action across all modalities, eliminating the time losses associated with sequential scanning and processing of different imaging types.
3Manufacturing precision
If precisely aligned image pairs are used for training, then accurate digital staining is achieved, but processing complexity and time increase
Solution Approach 1:
The system performs preliminary co-registration of multiple imaging modalities during the image acquisition phase itself. By synchronizing the imaging process and capturing all modalities simultaneously on the same tissue section, the alignment is established beforehand, eliminating the need for complex post-acquisition registration procedures.
Solution Approach 2:
The patent uses the tissue section itself as an intermediary reference for aligning multiple imaging modalities. Since all images are captured from the identical physical tissue section, the tissue morphology serves as a natural registration template, simplifying the alignment process compared to using separate tissue sections that would require complex computational matching.
4Ease of manufacture
If overlying sections are used for imaging, then staining and unstained images can be obtained, but morphological differences cause misalignment and artifacts
Solution Approach 1:
The system performs preliminary co-registration of multiple imaging modalities during the image acquisition phase itself. By synchronizing the imaging process and capturing all modalities simultaneously on the same tissue section, the alignment is established beforehand, eliminating the need for complex post-acquisition registration procedures.
Solution Approach 2:
The patent uses the tissue section itself as an intermediary reference for aligning multiple imaging modalities. Since all images are captured from the identical physical tissue section, the tissue morphology serves as a natural registration template, simplifying the alignment process compared to using separate tissue sections that would require complex computational matching.
Data Source
AI summary
In one aspect, the invention concerns an imaging method for generating a digitally stained image (51′) of a biological tissue probe (50) from a physical image (50′) of an unstained tissue biological tissue probe (50) in which a physical image (50′) of a biological tissue probe (50) is obtained by simultaneous multi-modal microscopy (53). In another aspect, the invention pertains to a training method for training an artificial intelligence system to be used in such a method. Moreover, the invention pertains to a system for generating a digitally stained image (51′) of a biological tissue probe (50) and/or for training an artificial intelligence system. The system comprises a processing unit for performing at least one of said methods. Furthermore, the invention relates to a non-transitory storage medium containing instructions that, when executed by a computer, causes the computer to perform said methods.