Multi-mode Imaging Optical System for Microparticle Analysis
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Solution Overview
Problem
Current methods for microparticle analysis, such as flow cytometry and fluorescence microscopy, are costly, complex, and limited in functionality, particularly in microfluidic chips where they lack the ability to perform multiple imaging modes without manual component switching and do not support scattered light imaging.
Innovation Solution
A multi-mode imaging optical system that includes absorption and forward scattering, side scattering, and fluorescent illumination units, arranged at specific angles with respect to the sample stage, allowing for in-situ imaging of absorption, forward scattered light, side scattered light, and fluorescence imaging without mechanical component switching.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If flow cytometry is used for microparticle detection, then detection capability is improved, but device complexity and cost increase significantly
Solution Approach 1:
The system segments detection functions into separate illumination units (transmitted light, reflected light, fluorescence) that can be independently activated. Each unit handles a specific detection mode, avoiding the need for a single complex system to perform all functions simultaneously.
Solution Approach 2:
The imaging system is designed to perform multiple detection functions (absorption imaging, scattered light imaging, fluorescence imaging) using a unified optical platform with switchable illumination units, replacing the need for separate specialized equipment.
2Adaptability or versatility
If fluorescence microscope is used for microparticle detection, then imaging function is improved, but operational complexity increases due to manual component switching
Solution Approach 1:
The illumination units are designed to be dynamically switchable between different detection modes (transmitted light, reflected light, fluorescence) without requiring manual reconfiguration of optical components. The system transitions between modes through electronic control rather than mechanical switching.
Solution Approach 2:
The system automatically manages the switching between different illumination units and detection modes without requiring manual intervention. The control system handles mode transitions, light source activation, and detector configuration autonomously.
3Device complexity
If simple detection equipment is used for absorption or fluorescence imaging, then device complexity is reduced, but functional versatility is limited
Solution Approach 1:
The system merges multiple simple detection capabilities (absorption imaging, scattered light imaging, fluorescence imaging) into a single integrated platform. Different illumination units and detection modes are combined in one system, achieving versatility without proportionally increasing complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables cost-effective, simple, and versatile in-situ imaging of microparticles in various modes, facilitating characteristic analysis and classification without the need for complex equipment maintenance, applicable in scientific research, clinical examinations, and industrial tests.
Implementation Method 1
an absorption and forward scattering illumination unit, disposed coaxially with the stage and the imaging unit in turn and configured to irradiate the to-be-tested sample, and form absorption imaging or forward scattered light imaging in the imaging unit
Implementation Method 2
form absorption imaging or forward scattered light imaging
Implementation Method 3
a side scattering illumination unit, forming a first given angle with the stage and configured to perform first oblique illumination on the to-be-tested sample, so that scattered light of microparticles in the to-be-tested sample forms side scattered light imaging in the imaging unit
Implementation Method 4
a fluorescent illumination unit, forming a second given angle with the stage and configured to perform second oblique illumination on the to-be-tested sample, and excite the microparticles in the to-be-tested sample to emit fluorescence
Data Source
AI summary
The present invention discloses a multi-mode imaging optical system. The multi-mode imaging optical system includes a stage configured to hold a to-be-tested sample. An imaging unit implements in-situ imaging of the to-be-tested sample. An absorption and forward scattering illumination unit irradiates the to-be-tested sample, and forms absorption imaging or forward scattered light imaging in the imaging unit. A side scattering illumination unit performs a first oblique illumination on the to-be-tested sample, so that scattered light of microparticles in the to-be-tested sample forms side scattered light imaging in the imaging unit. A fluorescent illumination unit performs a second oblique illumination on the to-be-tested sample, and excites the microparticles in the to-be-tested sample to emit fluorescence, where the fluorescence forms fluorescence imaging in the imaging unit.