Multi-Module Fluorescent Microscope Eliminates Mechanical Switching
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Solution Overview
Problem
Conventional multi-fluorescent microscopes require mechanical movement and separate equipment to switch between light sources and filters, leading to high production costs, mechanical vibrations, and prolonged time for obtaining multiple fluorescent images.
Innovation Solution
A fluorescent microscope system with multiple light sources and dichroic filters that allow for simultaneous acquisition of multiple fluorescent images without mechanical movement, using a configuration of optical modules with excitation and radiation filters to irradiate and detect light across different wavelengths independently.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If mechanical movement is used to switch between light sources and filters, then multiple fluorescent images can be obtained, but production costs increase and mechanical vibrations occur
Solution Approach 1:
The patent divides the optical system into multiple independent optical modules, each dedicated to a specific wavelength range. Each module contains its own light source, excitation filter, dichroic mirror, and image acquisition unit, eliminating the need for mechanical switching between different components. This segmentation allows simultaneous operation of multiple channels without mechanical movement.
Solution Approach 2:
Each optical module is designed to handle a specific wavelength range universally, with integrated components that perform multiple functions within that channel. The modular design allows the system to acquire multiple fluorescent images simultaneously through parallel optical paths, making the system versatile without requiring mechanical switching equipment.
2Adaptability or versatility
If mechanical movement is used to replace light sources and filters, then different wavelengths can be irradiated, but time is consumed for replacement
Solution Approach 1:
The optical system is segmented into multiple independent modules, each optimized for a specific wavelength range. This allows simultaneous irradiation of the sample with multiple wavelengths through parallel optical paths, eliminating the sequential time required for mechanical replacement of light sources and filters.
Solution Approach 2:
Multiple light sources operate continuously and simultaneously in parallel optical modules, providing continuous multi-wavelength irradiation without interruption. The system maintains continuous useful action by acquiring multiple fluorescent images simultaneously rather than sequentially, eliminating downtime for component replacement.
3Adaptability or versatility
If separate electronic and mechanical equipment is used for switching, then light sources and filters can be replaced, but production costs increase
Solution Approach 1:
The system is divided into independent optical modules that can be manufactured separately and assembled. Each module is a self-contained unit with standardized components, simplifying manufacturing processes and reducing overall production costs compared to complex mechanical switching systems. The segmented design allows for easier quality control and assembly.
Solution Approach 2:
The patent replaces mechanical switching systems with a static multi-module optical configuration. Instead of using motors, actuators, and mechanical switchers to change light sources and filters, the system uses fixed optical modules with integrated components, eliminating the need for separate mechanical switching equipment and reducing production costs.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the rapid and simple observation of multiple fluorescent images without mechanical movement, reducing production costs and minimizing image acquisition time.
Implementation Method 1
a plurality of dichroic filters disposed to be spaced apart from each other, to reflect only light having a wavelength in a selected range of the radiation light radiated from the survey object, respectively
Implementation Method 2
A fluorescent microscope refers to an apparatus which processes a fluorescent material (fluorescent pigment) in a sample by using a principle in which fluorescent light is emitted from a fluorescent substance if the fluorescent substance absorbs light having a specific wavelength
Data Source
AI summary
A fluorescent microscope for observing multiple fluorescent images includes: a first optical module comprising a first light source for supplying first excitation light having a first wavelength, a first excitation filter for selectively transmitting the first excitation light supplied from the first light source, a first dichroic filter for reflecting the first excitation light having passed through the first excitation filter toward the survey object, an objective lens for condensing the first excitation light reflected by the first dichroic filter and transferring the condensed first excitation light to the survey object, a second dichroic filter for reflecting first radiation light radiated from the survey object, a first radiation filter for selectively transmitting the first radiation light reflected by the second dichroic filter, and a first image acquisition unit for acquiring an image by using the first radiation light having passed through the first radiation filter to be supplied; and a second optical module comprising a second light source for supplying second excitation light having a second wavelength, a second excitation filter for selectively transmitting the second excitation light supplied from the second light source, a second radiation filter passing through the second excitation filter and irradiated to the survey object to be radiated, to selectively transmitting the second radiation light EM2 having passed through the objective lens, the first dichroic filter, and the second excitation filter, and a second image acquisition unit for acquiring an image by using the second radiation light having passed through the second radiation filter to be supplied.