Multi-Peptide Recombinant Protein Expression for Accurate Low-Cost Production
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Solution Overview
Problem
Existing functional peptides are single-function and costly to manufacture, and simultaneous production of multiple peptides with accurate amino acid sequences is difficult, limiting their effectiveness in treating and preventing diseases.
Innovation Solution
A recombinant protein comprising multiple multi-peptide sets is developed, where each set is inserted into a carrier protein using sequencing primers, and expressed in a yeast system to enhance functional peptide concentrations and synergistic effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple functional peptides are manufactured through artificial synthesis, then therapeutic effectiveness is improved, but manufacturing cost increases and manufacturing precision deteriorates
Solution Approach 1:
The patent combines multiple functional peptides into a single recombinant protein molecule, where different peptide sequences are integrated into one expression system. This allows simultaneous production of multiple peptides through a single manufacturing process, reducing overall cost while maintaining therapeutic effectiveness through synergistic peptide combinations
Solution Approach 2:
The recombinant protein serves multiple functions by incorporating different functional peptide sequences that can address various therapeutic needs. A single recombinant protein product can provide multiple therapeutic effects, reducing the need for separate manufacturing processes for each peptide
2Reliability
If multiple functional peptides are manufactured through artificial synthesis, then therapeutic effectiveness is improved, but manufacturing precision deteriorates
Solution Approach 1:
The patent replaces complex chemical synthesis processes with biological expression systems. By using recombinant DNA technology and expressing peptides in living cells, the system achieves higher precision in amino acid sequence accuracy while maintaining the ability to produce multiple functional peptides with correct sequences
3Adaptability or versatility
If multiple peptides are produced simultaneously through artificial synthesis, then functional versatility is improved, but device complexity increases
Solution Approach 1:
The patent merges multiple peptide production processes into a single recombinant protein expression system. By integrating multiple peptide sequences into one protein molecule, the system achieves functional versatility while simplifying the manufacturing process to a single expression and purification step
4Quantity of substance
If peptide concentration is increased through simultaneous production, then therapeutic effectiveness is improved, but manufacturing precision deteriorates
Solution Approach 1:
The patent replaces artificial synthesis with biological expression to achieve high peptide concentrations while maintaining sequence accuracy. The living expression system naturally ensures correct amino acid incorporation even at high production levels, overcoming the precision limitations of chemical synthesis at scale
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant protein provides comprehensive therapeutic effects by combining multiple functional peptides, demonstrating improved treatment outcomes for conditions like sleep disorders and diabetes.
Implementation Method 1
transferred into a yeast expression system for fermentation
Implementation Method 2
purifying the post-fermentation recombinant protein using starch
Data Source
AI summary
A recombinant protein comprising multiple multi-peptide sets includes first through fifth sequencing primers and first through fourth multi-peptide regions. The first multi-peptide region is between the first and the second sequencing primers. The second multi-peptide region is between the second and the third sequencing primers. The third multi-peptide region is between the third and the fourth sequencing primers. The fourth multi-peptide region is between the fourth and the fifth sequencing primers. In each of the multi-peptide regions, multiple functional peptides can be inserted, and manufacturing thereof can be done through expression of the recombinant protein, thereby significantly enhancing the concentrations of the functional peptides. With the combination of peptide having different functions, the recombinant protein product can provide more complete and more comprehensive functionality. This application also discloses a pharmaceutical composition comprising the recombinant protein and a method for preparing the recombinant protein.