Multi-Band Fluorescence Imaging for Single-Path Signal Acquisition
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Solution Overview
Problem
Existing fluorescence imaging devices struggle with complex structures and high costs when detecting multiple fluorescence signals, making it difficult to acquire multi-fluorescence images simultaneously.
Innovation Solution
A system comprising an excitation light source, optical detection device, optical lens, and multi-band filter, which allows for the simultaneous acquisition of multi-fluorescence images using a single excitation light source and optical detection device, with the multi-band filter filtering and converging fluorescence signals to generate clear images.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple detection light paths are used to acquire multi-fluorescence signals, then measurement precision is improved, but device complexity increases
Solution Approach 1:
The patent combines multiple detection light paths into a single detection path by using a multi-band filter that can simultaneously transmit multiple wavelength intervals. Instead of having separate detection paths for each fluorescence signal, the system merges them into one path where the multi-band filter separates and transmits different wavelength ranges through a single optical detection device, thereby reducing device complexity while maintaining measurement precision.
Solution Approach 2:
The single optical detection device is designed to perform multiple functions by detecting different wavelength intervals simultaneously. The multi-band filter enables the detection device to universally capture multiple fluorescence signals (e.g., green and red fluorescence) through a single device, eliminating the need for multiple specialized detection paths and reducing overall system complexity.
2Measurement precision
If repeated irradiation is used to acquire multiple fluorescence signals, then measurement precision is improved, but loss of energy increases and time is consumed
Solution Approach 1:
The patent applies continuous irradiation with a single excitation light source while the multi-band filter simultaneously transmits multiple wavelength intervals. This allows the system to continuously acquire multiple fluorescence signals without interrupting the excitation process, eliminating the need for repeated irradiation sequences. The useful action of detecting multiple signals continues simultaneously throughout the irradiation process, reducing energy loss and time consumption compared to sequential repeated detection.
3Measurement precision
If repeated detection is used to form multi-fluorescence images, then measurement precision is improved, but loss of time increases and productivity decreases
Solution Approach 1:
The patent merges multiple detection operations into a single simultaneous detection event. By using the multi-band filter to transmit multiple wavelength intervals through one detection path, the system acquires multiple fluorescence images at the same time rather than sequentially through repeated detections. This merging of detection operations dramatically increases productivity while maintaining image quality through simultaneous multi-signal capture.
4Adaptability or versatility
If multiple detection light paths are used, then adaptability is improved, but device complexity increases and cost increases
Solution Approach 1:
The single optical detection device achieves universality by detecting multiple wavelength intervals simultaneously through the multi-band filter. The device can adapt to detect different fluorescence signals (e.g., various colors and wavelengths) through a single unified structure, eliminating the need for multiple specialized detection paths. This multi-functional design maintains high adaptability while significantly reducing device complexity and cost.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system simplifies the structure and reduces costs, enabling efficient and effective acquisition of multi-fluorescence images without the need for multiple detection paths, improving imaging quality and efficiency.
Implementation Method 1
The multi-band filter may be configured to filter the first multi-fluorescence signal to generate a second multi-fluorescence signal. The multi-band filter may have at least two transmission intervals corresponding to at least two wavelength intervals.
Implementation Method 2
The optical lens may be configured to converge the first multi-fluorescence signal to the optical detection device.
Implementation Method 3
samples containing fluorescent labels (e.g., cells labeled with fluorescent dyes, etc.) may emit fluorescence signals under the irradiation of an excitation light source
Data Source
AI summary
A system for acquiring multi-fluorescence images includes an excitation light source configured to irradiate an excitation beam onto a surface of a test sample, so that the test sample emits multi-fluorescence signals. The multi-fluorescence signals include at least two fluorescence signals. The system also includes an optical detection device configured to acquire the multi-fluorescence signals, an optical lens disposed between the optical detection device and the test sample, and a multi-band filter disposed between the optical detection device and the optical lens and/or between the test sample and the optical lens. The optical lens is configured to converge the multi-fluorescence signals emitted by the test sample to the optical detection device. The multi-band filter has at least two transmission intervals corresponding to at least two wavelength intervals of the at least two fluorescence signals. The optical detection device is configured to generate multi-fluorescence images based on the multi-fluorescence signals.