Multichromophore UV Absorption for Diagnostic Sensitivity
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Solution Overview
Problem
Current fluorescent dyes for molecular recognition and labeling in biochemistry and medicine lack efficient methods for targeting specific biomolecules with high specificity and sensitivity, particularly in terms of ultraviolet absorption and emission profiles, which limits their application in diagnostic and research tools.
Innovation Solution
Development of water-soluble light harvesting multichromophores with a conjugated segment including a fused 6-5-6 tricyclic co-monomer and a UV absorbance-modifying co-monomer, along with an acceptor chromophore covalently linked in energy-receiving proximity, to enhance ultraviolet absorption and emission properties, and the incorporation of a specific binding member for targeted molecular interactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional fluorescent dyes are used for molecular recognition, then the dyes can be applied in biochemistry and biology, but the sensitivity and specificity for target biomolecule detection are limited
Solution Approach 1:
The patent employs composite fluorescent probes consisting of a fluorescent dye covalently linked to a specific binding member (such as antibodies, aptamers, or peptides). This composite structure combines the optical properties of the dye with the target-specific recognition capabilities of the binding member, thereby simultaneously improving detection sensitivity/specificity and maintaining broad adaptability across different diagnostic applications.
Solution Approach 2:
The specific binding member acts as an intermediary that mediates between the fluorescent dye and the target biomolecule. The binding member provides high-affinity, specific recognition of the target, while the fluorescent dye serves as the signaling reporter, enabling enhanced detection capabilities without limiting the range of applicable diagnostic tools.
2Use of energy by moving object
If fluorescent dyes with ultraviolet absorption are used, then energy transfer efficiency can be improved, but the background fluorescence and penetration depth are compromised
Solution Approach 1:
The patent utilizes the local quality principle by selecting fluorescent dyes with specific absorption and emission wavelength characteristics optimized for particular application needs. Different dye molecules with distinct spectral properties can be chosen to match the excitation sources and detection systems of specific diagnostic tools, allowing energy transfer efficiency to be maximized locally for each application while managing background fluorescence and penetration depth according to the specific requirements of that application.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The multichromophores provide improved ultraviolet absorption and emission profiles, enabling more efficient energy transfer and amplification, thereby enhancing the sensitivity and specificity of molecular recognition and labeling processes, leading to better diagnostic and research outcomes.
Implementation Method 1
Water soluble light harvesting multichromophores that have an ultraviolet absorption maximum
Implementation Method 2
an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith
Data Source
AI summary
Water soluble light harvesting multichromophores that have an ultraviolet absorption maximum are provided. In some embodiments, the multichromophores include a conjugated segment including a fused 6-5-6 tricyclic co-monomer and a UV absorbance-modifying co-monomer. The multichromophores may include an acceptor chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. In some embodiments, a specific binding member is covalently linked to the multichromophore. Also provided are methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule using compositions including the light harvesting multichromophores. Kits and systems for practicing the subject methods are also provided.