Multicolor Fluorescence Analysis Device Using Single Dielectric Filter
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Solution Overview
Problem
Existing multicolor fluorescence analysis devices require extensive time for analysis due to the need to replace interference filters for each fluorescent pigment, limiting the speed of base sequence analysis in methods like SBS.
Innovation Solution
A multicolor fluorescence analysis device using a single dielectric multilayer film interference filter and a two-dimensional detection unit, which collectively detects fluorescent light from multiple fluorescent pigments by filtering out excitation light and transmitting specific wavelength bands, allowing for simultaneous detection of Alexa405, FAM, Texas Red, and Cy5.5.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a replaceable interference filter is used for each fluorescent pigment, then the detection accuracy of specific wavelength fluorescent light is improved, but the analysis time increases significantly due to the need to replace filters for each base extension reaction
Solution Approach 1:
A single dielectric multilayer film interference filter is designed to perform multiple functions by simultaneously transmitting multiple excitation wavelength bands (violet 380-400nm, blue 450-480nm, green 500-550nm, red 600-680nm) and blocking corresponding fluorescent light wavelengths. This multi-functional filter eliminates the need for multiple replaceable filters, enabling parallel detection of multiple fluorescent pigments (FAM, HEX, ROX, Cy5) and significantly reducing analysis time while maintaining detection accuracy
2Productivity
If multiple excitation wavelength bands are used simultaneously, then the detection of multiple fluorescent pigments is accelerated, but the excitation light may overlap with fluorescent light wavelengths causing detection interference
Solution Approach 1:
The dielectric multilayer film interference filter acts as an intermediary component between the excitation light source and the fluorescent samples. It selectively transmits multiple excitation wavelength bands while simultaneously blocking the corresponding fluorescent light wavelengths from reaching the detector. This intermediary filtering mechanism prevents excitation light overlap with fluorescent light, eliminating detection interference and ensuring accurate fluorescence signal detection while enabling simultaneous multi-color detection
Solution Approach 2:
The interference filter is designed with specific transmission and blocking parameter characteristics for different wavelength bands. By precisely controlling the transmission wavelengths (excitation bands) and blocking wavelengths (fluorescence bands), the system achieves optimal separation between excitation and emission light. This parameter optimization allows simultaneous use of multiple excitation wavelengths without spectral overlap, enabling high-speed multi-color fluorescence detection with maintained accuracy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate detection of multiple fluorescent pigments, improving analysis speed and efficiency in base sequence analysis, such as DNA sequencing, by suppressing excitation light overlap and enhancing fluorescence detection accuracy.
Implementation Method 1
a single dielectric multilayer film interference filter which is disposed on an end surface of the second exiting portion, and transmits at least a part of the fluorescent light and transmits light having a plurality of transmission wavelength bands not including the excitation wavelength band
Implementation Method 2
a first fiber optic plate which is in contact with at least a part of the sample, receives light including the fluorescent light emitted from the sample by being irradiated with the excitation light from a first incident portion and guides the light
Implementation Method 3
detecting fluorescent light which is emitted from a plurality of types of fluorescent pigments having different fluorescence wavelengths contained in a sample by being irradiated with excitation light
Data Source
AI summary
A multicolor fluorescence analysis device is provided. The device includes a first fiberoptic plate for guiding light including fluorescence emitted from a sample as a result of irradiation of excitation light and emitting the same from a first emission part. The device includes a second fiberoptic plate for receiving light emitted from the first emission part at a second incidence part, guiding the same, and emitting the same from a second emission part. The device includes a single multilayer dielectric interference film filter that is provided on an end surface of the second emission part, transmits at least a portion of the fluorescence, and transmits light of a plurality of transmission wavelength bands that do not include the excitation wavelength bands. The device includes a two-dimensional detection unit that is disposed so as to be adhered to the multilayer dielectric interference film filter.