Multilayered Vesicle Assembly Without High-Shear Biomolecule Damage
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Solution Overview
Problem
Existing methods for forming multilamellar lipid vesicles are imprecise and can degrade fragile biomolecules due to high-energy shear, limiting the development of robust, layered vesicles suitable for drug delivery applications.
Innovation Solution
A method involving the addition of hydrophobe containing polypeptoid (HCP)-lipid complexes to unilamellar vesicles to form multilayered vesicles, which are more robust and maintain structural integrity without high-energy shear.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Shape
If high energy shear is used to form multilamellar vesicles, then multilayered structure is achieved, but fragile biomolecules are degraded
Solution Approach 1:
The patent uses unilamellar vesicles as intermediary structures that serve as templates for multilayer formation. These pre-formed vesicles provide a stable core that protects biomolecules while allowing controlled assembly of additional layers through HCP-lipid complex addition, avoiding direct high-energy shear exposure of the cargo
Solution Approach 2:
The methodology performs preliminary formation of unilamellar vesicles with encapsulated cargo before proceeding to multilayer assembly. This sequential approach ensures that biomolecules are protected within the inner vesicle structure before outer layers are added, preventing degradation during the multilayer formation process
2Shape
If high energy shear is applied to create multilamellar vesicles, then layered structure is formed, but manufacturing precision is reduced
Solution Approach 1:
The patent segments the vesicle formation process into distinct stages: first forming unilamellar vesicles with controlled precision, then adding HCP-lipid complexes to form additional layers. This segmentation allows each stage to be optimized independently, maintaining manufacturing precision while achieving multilamellar structure
Solution Approach 2:
Unilamellar vesicles serve as precise intermediary structures that define the core geometry and size of the final multilamellar vesicles. By controlling the formation and characteristics of these intermediary vesicles, the patent maintains manufacturing precision throughout the multilayer assembly process
3Shape
If conventional methods are used to form multilamellar vesicles, then layered structure is achieved, but structural integrity is compromised
Solution Approach 1:
The patent implements a nested structure where unilamellar vesicles are embedded within multilamellar configurations formed by HCP-lipid complexes. This nesting creates a hierarchical architecture where the inner vesicle provides structural integrity while outer layers add stability and functionality
Solution Approach 2:
The methodology creates composite vesicle structures combining unilamellar vesicle membranes with HCP-lipid complex layers. This composite approach integrates the structural integrity of the inner vesicle with the stabilizing properties of the outer HCP-lipid layers, enhancing overall vesicle strength
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for the formation of multilayered vesicles with retained cargo and flexible, discontinuous layers, enhancing drug delivery capabilities by avoiding degradation and maintaining vesicle integrity.
Implementation Method 1
adding a plurality of hydrophobe containing polypeptoid (HCP)-lipid complexes to a plurality of unilamellar vesicles
Implementation Method 2
the HCP-lipid complexes comprise about 0.25 wt % lipid and 0.5 wt % HCP... the complex is formed by a physical association of the HCP with a lipid or a lipid bilayer fragment
Data Source
AI summary
The disclosure relates to multilayered vesicles, methods for forming multilayered vesicles, and drug delivery complexes including multilayered vesicles. The multilayered vesicles can be formed by adding hydrophobe containing polypeptoid (HCP)-lipid complexes to unilamellar vesicles such as liposomes in an amount effective to form multilayered vesicles. The HCP-lipid complexes can be produced by mixing a hydrophobe containing polypeptoid (HCP) with a first set of unilamellar vesicles. The unilamellar vesicles and/or drug delivery complexes can include various cargoes for delivery, such as water-soluble cargoes, hydrophobic cargoes, etc.