Multi-mode Signal Acquisition Device for Laser Imaging
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Solution Overview
Problem
Existing multimodal signal acquisition systems suffer from calibration deviations and impaired imaging effects due to overlapping spectral signals, particularly in nonlinear laser microscopy, where fluorescence and harmonic signals are difficult to separate effectively.
Innovation Solution
A multimodal signal acquisition device with independent-channel acquisition modules and spectral signal processing, utilizing multiple spectral signal acquisition channels and a deconvolution algorithm to separate FAD and NADH fluorescence signals, as well as harmonic signals, by applying a formula (T FAD = H FAD − a × H NADH ) to remove interference and achieve pure FAD fluorescence signals.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If spectral signals of different spectral ranges are acquired using a common acquisition device, then multiple nonlinear molecular image modes can be obtained, but the spectral information overlaps and is difficult to separate, introducing calibration deviation and affecting imaging effect
Solution Approach 1:
The patent divides the acquisition system into multiple independent acquisition channels, each dedicated to a specific spectral range. The signal acquisition device includes a first acquisition channel for harmonic signals and a second acquisition channel for fluorescence signals, with each channel having its own detector and spectral range settings. This segmentation prevents spectral overlap and eliminates calibration deviations between different spectral acquisitions.
2Loss of information
If spectral scanning is performed to acquire fluorescence emission spectra, then spectral imaging can be achieved, but linear unmixing is required which complicates the processing and may introduce errors
Solution Approach 1:
Instead of acquiring complete spectra and performing computational unmixing, the patent segments the detection into multiple channels with fixed spectral filters. Each channel detects a specific spectral component directly, eliminating the need for linear unmixing algorithms and reducing processing complexity while preserving spectral information.
3Measurement precision
If multiple signal channels are used to detect different spectral components, then spectral separation is improved, but the device structure becomes more complex
Solution Approach 1:
The patent implements independent acquisition channels for different spectral ranges, with each channel containing appropriate filters and detectors. This segmentation achieves precise spectral separation by physically isolating different wavelength components before detection, rather than relying on post-acquisition computational methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution ensures effective separation of spectral signals, making molecular or structural information orthogonal in the image, thereby enhancing the imaging performance of laser systems by avoiding calibration deviations and improving signal clarity.
Implementation Method 1
The multimodal signal includes spectral signals of different spectral ranges, including a harmonic signal of the laser pulse and a fluorescence signal
Implementation Method 2
The multimodal signal includes spectral signals of different spectral ranges, including a harmonic signal of the laser pulse
Data Source
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AI summary
A multimodal signal acquisition device and method, and a laser image system are provided. The multimodal signal acquisition device is configured to acquire a multimodal signal generated by a laser pulse that irradiates a sample and includes an independent-channel acquisition module and a spectral signal processing device. The independent-channel acquisition module is provided with multiple independent spectral signal acquisition channels, each of which corresponds to a spectral signal of a specific spectral range in the multimodal signal. Each of the spectral signal acquisition channels acquires the multimodal signal, filters the corresponding spectral signal of the specific spectral range from the multimodal signal, and sends the spectral signal to the spectral signal processing device. The spectral signal processing device is configured to receive the spectral signal acquired by each of the spectral signal acquisition channels and perform imaging and superposed output of each spectral signal.