Multiphase Polymer Janus Particle for Rapid Biological Detection
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Solution Overview
Problem
Conventional methods for detecting biological specimen substances, such as ELISA and radioimmunoassay, are complex and time-consuming, necessitating a more efficient and accurate detection technique.
Innovation Solution
Development of nano-sized multiphase polymer Janus particles with one surface modified to bind specimen substances and the other with labeling substances, combined with a chip device for immobilizing capturing reagents, enabling quick and accurate detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional ELISA or radioimmunoassay methods are used for detecting specimen substances, then detection can be performed, but the operation process becomes complicated and time-consuming
Solution Approach 1:
The particle is divided into two distinct surfaces with different functions: one surface is modified with capturing reagents for binding specimen substances, while the other surface is modified with labeling substances for detection. This segmentation allows simultaneous presence of both functional groups on a single particle, eliminating the need for separate操作步骤 and reducing process complexity while maintaining detection accuracy
Solution Approach 2:
The invention merges the capturing function and labeling function into a single particle structure. By combining both functional surfaces on one particle, the method integrates multiple detection steps into a unified system, simplifying the operation process while preserving measurement precision
2Measurement precision
If conventional ELISA or radioimmunoassay methods are used for detecting specimen substances, then detection can be performed, but the detection process becomes time-consuming
Solution Approach 1:
Both capturing reagents and labeling substances are pre-modified onto the particle surfaces before detection. This preliminary preparation allows the particle to be ready for immediate use, eliminating the need for sequential addition of reagents during the detection process and significantly reducing detection time while maintaining accuracy
Solution Approach 2:
By combining capturing and labeling functions in a single particle, the invention allows simultaneous performance of binding and detection functions, reducing the number of sequential steps and overall detection time while preserving measurement precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The multiphase polymer Janus particle-based sensor enhances detection sensitivity and accuracy for biological specimen substances, facilitating disease diagnosis and virus/pathogen examination.
Implementation Method 1
one surface modified to bind specimen substances
Implementation Method 2
the other with labeling substances
Data Source
Figure 1
Figure 2A~2B
Figure 3A~3C
AI summary
An object of the present invention is to provide an improved detection method for a specimen substance in a sample. The present invention provides a multiphase polymer fine particle having two or more polymer phases formed by aggregation of two or more polymers, respectively, at least on a surface thereof, wherein a first polymer phase can bind to a specimen substance or a substance that can specifically bind to the specimen substance, and a second polymer phase has a labeling substance. By utilizing the fine particle, the specimen substance in the sample having a biological origin or the like can be quickly detected with high sensitivity.