Multiplex Coronavirus Detection Composition
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Solution Overview
Problem
Current methods lack the capability for simultaneous and rapid identification and typing of multiple coronaviruses, particularly the highly contagious and harmful novel coronavirus 2019-nCoV, MERSr-CoV, and SARSr-CoV, which hinders precise diagnosis and control measures.
Innovation Solution
A composition comprising specific primers and probes for each coronavirus, including 2019-nCoV, NL63, HKU1, 229E, OC43, MERSr-CoV, and SARSr-CoV, used in a fluorescent probe method and melting curve analysis to enable simultaneous detection and typing in a single tube, reducing time and costs while preventing false positives and environmental contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple separate detection methods are used for different coronaviruses, then detection accuracy for each virus can be maintained, but detection time increases and operational complexity increases
Solution Approach 1:
The patent combines multiple coronavirus detection assays into a single multiplex RT-PCR reaction system. Different coronavirus targets (2019-nCoV, SARSr-CoV, MERSr-CoV, and other coronaviruses) are detected simultaneously in one reaction tube using virus-specific primers and fluorescently labeled probes, each emitting at distinct wavelengths. This merging approach maintains individual detection accuracy while reducing total detection time and operational steps compared to performing separate assays for each coronavirus.
Solution Approach 2:
The invention creates a universal detection platform that can identify multiple coronavirus types simultaneously. The reaction system uses a common RT-PCR master mix and standardized protocol, but incorporates virus-specific primers and probes to detect different coronaviruses. This multi-functional system allows a single test to screen for various coronaviruses, eliminating the need for multiple separate detection methods and reducing both time and operational complexity.
2Measurement precision
If multiple separate detection methods are used for different coronaviruses, then detection accuracy for each virus can be maintained, but operational complexity increases
Solution Approach 1:
The patent merges multiple coronavirus detection procedures into a single multiplex RT-PCR assay. All necessary reagents (reverse transcriptase, DNA polymerase, primers, probes, buffers) are combined in one reaction tube, and a single amplification cycle detects multiple coronavirus targets simultaneously. This reduces operational complexity by eliminating the need to set up, execute, and analyze multiple separate assays while preserving detection accuracy through virus-specific primers and probes.
Solution Approach 2:
The invention develops a universal coronavirus detection system that handles multiple virus types through a standardized workflow. The system uses a common reaction protocol and data analysis method, with virus identification achieved through fluorescent channel analysis. This universal approach simplifies operations compared to multiple separate assays, as personnel need to master only one detection method that can identify various coronaviruses.
3Adaptability or versatility
If multiple separate detection methods are used for different coronaviruses, then comprehensive coronavirus identification can be achieved, but time consumption and costs increase
Solution Approach 1:
The patent combines comprehensive coronavirus identification capabilities into a single multiplex detection system. The reaction includes primers and probes for 2019-nCoV, SARSr-CoV, MERSr-CoV, and other coronaviruses, all detected simultaneously in one assay. This merging provides comprehensive identification coverage while reducing time consumption compared to performing sequential separate assays for each coronavirus type.
4Adaptability or versatility
If multiple separate detection methods are used for different coronaviruses, then comprehensive coronavirus identification can be achieved, but costs increase
Solution Approach 1:
The patent merges comprehensive coronavirus detection into one reaction tube, reducing reagent consumption. Instead of using separate volumes of RT-PCR reagents, primers, and probes for each coronavirus assay, the multiplex system uses a single set of reagents that detects multiple viruses simultaneously. This conserves expensive reagents while maintaining comprehensive identification capability across different coronavirus types.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for efficient detection and typing of eight targets in one test, improving detection efficiency, reducing operational complexity, and ensuring accurate results without crossover contamination, thereby facilitating timely infection control and prevention.
Implementation Method 1
fluorescent probe method and melting curve analysis
Implementation Method 2
fluorescent probe method and melting curve analysis
Data Source
AI summary
Provided in the present invention is a composition capable of detecting and typing novel coronavirus 2019-nCoV, coronavirus 229E, coronavirus NL63, coronavirus OC43, coronavirus HKU1, coronavirus MERSr-CoV, and coronavirus SARSr-CoV. At the same time, further provided is a kit comprising the composition and a method for detecting and typing coronaviruses. The composition of the present invention in combination with a fluorescent probe method and a melting curve method can perform simultaneous detection and typing of seven coronaviruses in one tube.