Multiplex ELISA Substrate for Bovine Pathogen Screening
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Solution Overview
Problem
Current veterinary diagnostic tests for bovine infectious diseases, such as IBR, BVD, neosporosis, Johne's disease, and leptospirosis, are time-consuming, expensive, and often require large sample volumes, limiting their effectiveness in rapid and accurate detection, especially for identifying latent infections and differentiating between infected and vaccinated animals.
Innovation Solution
A multiplex method and substrate for screening bovine samples that simultaneously detect antibodies against multiple pathogens, including BVDV, BoHV-1, Mycobacterium paratuberculosis, Leptospira species, Neospora caninum, and Fasciola hepatica, using immobilized antigens like NS3, Erns, gB, gE, LipL32, SRS2, SAG1, and Cathepsin L1, allowing for simultaneous detection in a single assay, and eliminating the need for pre-absorption steps, thereby enhancing efficiency and accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If singleplex ELISA is used for serological detection of bovine infectious diseases, then diagnostic accuracy for individual pathogens is maintained, but testing time and cost increase significantly
Solution Approach 1:
The patent combines multiple singleplex ELISA tests into a single multiplex ELISA assay that detects antibodies against multiple bovine pathogens (IBR, BVD, neosporosis, Johne's disease, leptospirosis) simultaneously. This merging of separate diagnostic procedures into one integrated test reduces total testing time while maintaining diagnostic accuracy through pathogen-specific antigen arrays and optimized detection protocols
Solution Approach 2:
The invention creates a universal diagnostic platform that can detect multiple different pathogens using a single ELISA system. The multiplex assay uses a common microplate format with different antigen coatings to achieve multi-functionality, allowing one test system to perform the work of multiple separate tests while maintaining pathogen-specific detection accuracy
2Measurement precision
If singleplex ELISA is used for each pathogen, then specific pathogen detection is achieved, but sample volume requirements and cost increase
Solution Approach 1:
The patent merges multiple pathogen detection assays into a single multiplex ELISA that processes one sample volume against multiple pathogen antigens simultaneously. This approach maintains the specificity needed for individual pathogen detection while reducing the total sample volume required compared to running separate singleplex tests for each pathogen
Solution Approach 2:
The multiplex ELISA system serves as a universal detection platform that can identify multiple pathogens from a single sample aliquot. The system maintains pathogen-specific detection capability through specialized antigen arrays while optimizing sample utilization across all detections
3Measurement precision
If traditional diagnostic methods are used, then individual pathogen identification is accurate, but disease outbreak response speed decreases
Solution Approach 1:
The patent combines multiple pathogen detection capabilities into a single rapid multiplex ELISA assay that can screen for five different bovine diseases simultaneously. This integration maintains accurate pathogen identification while significantly accelerating outbreak response by eliminating the sequential testing required by traditional methods
Solution Approach 2:
The multiplex ELISA system performs preliminary screening for multiple pathogens in a single test, allowing rapid identification of affected herds and enabling faster implementation of control measures. The assay provides comprehensive pathogen profile data upfront, eliminating the need for sequential follow-up tests
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The multiplex method enables fast, cost-effective, and accurate detection of multiple pathogens, facilitating better disease control by identifying infected animals, differentiating between infected and vaccinated individuals, and reducing the risk of disease spread within herds, while also providing normalized results for easier interpretation.
Implementation Method 1
A multiplex method and substrate for screening bovine samples that simultaneously detect antibodies against multiple pathogens, including BVDV, BoHV-1, Mycobacterium paratuberculosis, Leptospira species, Neospora caninum, and Fasciola hepatica, using immobilized antigens
Data Source
AI summary
The current invention provides a multiplex method for screening bovine samples for antibodies against several important pathogens. These pathogens include Bovine Viral Diarrhoea Virus (BVDV), Bovine Herpesvirus-1 (BoHV-1), Mycobacterium paratuberculosis (MAP), Leptospira species, Neospora caninum and Fasciola hepatica. This multiplex screening is enabled by substrates with immobilized pathogen antigens and offer multiple advantages for the routine testing of farm animals.