Multiplex Oligomer Detection for Adenovirus, Metapneumovirus, and Rhinovirus
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Solution Overview
Problem
Current methods for detecting adenovirus, metapneumovirus, and rhinovirus are limited by their inability to rapidly, sensitively, and specifically identify multiple serotypes and subtypes, often requiring time-consuming and expensive processes that need skilled technicians.
Innovation Solution
The use of specific first and second amplification oligomers targeting defined nucleotide positions within adenovirus, metapneumovirus, and rhinovirus genomes to amplify and detect these pathogens with high sensitivity, utilizing nucleic acid amplification techniques.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If conventional detection methods (serological tests and cell culture) are used for adenovirus, then detection can be performed with simple equipment, but the detection speed is slow and sensitivity is insufficient
Solution Approach 1:
The patent replaces conventional mechanical detection methods (cell culture, serological tests) with a molecular biology-based detection system using PCR amplification and hybridization probes. This substitution enables rapid detection within hours rather than days or weeks, while maintaining manageable system complexity through standardized reagent kits
Solution Approach 2:
The patent introduces nucleic acid amplification intermediates (amplicons) and hybridization probes as mediators between the target adenovirus genome and the detection system. These intermediaries enable specific and sensitive detection by amplifying viral DNA sequences and providing targeted hybridization signals, resolving the contradiction between speed and complexity
2Adaptability or versatility
If PCR-based assays with low stringency conditions are used to detect genetically diverse adenoviruses, then detection coverage is improved, but measurement precision decreases
Solution Approach 1:
The patent applies local quality by designing hybridization probes that target specific conserved regions within the adenovirus genome while maintaining high stringency conditions. Each probe is optimized for its specific target region, enabling precise discrimination between adenovirus serotypes even while detecting genetically diverse strains. This resolves the contradiction by making different parts of the detection system (probes targeting different regions) have specialized properties
Solution Approach 2:
The patent changes the stringency parameter of hybridization conditions to achieve both broad coverage and high precision. By optimizing hybridization temperature, salt concentration, and probe length, the system maintains high stringency for specific detection while the set of probes collectively covers diverse adenovirus serotypes through targeted design
3Measurement precision
If multiple separate detection methods are used for different viruses, then detection accuracy for each virus is maintained, but the complexity of the overall detection process increases
Solution Approach 1:
The patent creates a universal detection platform that can detect multiple respiratory viruses (adenovirus, metapneumovirus, rhinovirus) using the same basic methodology of nucleic acid amplification and hybridization. Different virus-specific probes are used within the same reaction system, maintaining detection accuracy for each virus while reducing overall process complexity compared to separate detection methods for each pathogen
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, sensitive, and specific detection of multiple adenovirus serotypes, metapneumovirus subtypes, and rhinovirus strains, reducing the need for skilled labor and lowering costs while improving diagnostic accuracy.
Implementation Method 1
The oligomers are complementary to a target sequence
Implementation Method 2
utilizing nucleic acid amplification techniques
Data Source
AI summary
The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.