Multiplex Virus Detection on Solid-Phase Primers Across Species
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Solution Overview
Problem
Current virus detection methods in cell therapy are limited to detecting viruses from the same species and face challenges such as nonspecific binding, variability in amplification efficiency, and suboptimal reaction conditions, necessitating a need for a method that can efficiently and accurately detect multiple viruses from different species simultaneously.
Innovation Solution
A method involving coating nucleic acid primers corresponding to each virus on a solid-phase carrier, setting specific amplification reaction conditions, and conducting an amplification reaction to detect multiple viruses from different species, including optimizing reaction conditions for sensitivity and accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiplex PCR technology is used to detect multiple viruses simultaneously, then detection efficiency is improved, but nonspecific binding between primers and variability in amplification efficiency occur
Solution Approach 1:
The patent applies parameter changes by optimizing specific reaction conditions including setting the amplification reaction temperature below 70°C, primer length between 14-30 mer, primer concentration between 0.1-1 μM, and cycle number less than 40. These parameter adjustments resolve the contradiction by creating optimal conditions that enable simultaneous detection of multiple viruses while maintaining specific binding and consistent amplification efficiency across all target viruses.
2Measurement precision
If current virus detection methods are used, then detection of single virus or limited viruses from same species is achieved, but applicability to viruses from different species is restricted
Solution Approach 1:
The patent implements universality by designing a multiplex PCR system that can detect multiple viruses from different species simultaneously in a single reaction. The method uses a set of primers that can amplify target nucleic acids from human, bovine, and porcine viruses, making the detection system versatile across different species while maintaining detection accuracy through optimized reaction conditions.
3Measurement precision
If trial-and-error testing is used to optimize reaction conditions, then detection accuracy can be improved, but cost and time consumption increase
Solution Approach 1:
The patent applies preliminary action by pre-optimizing the amplification reaction conditions before actual virus detection. The optimized parameters (temperature below 70°C, primer length 14-30 mer, concentration 0.1-1 μM, cycles <40) are established in advance through systematic optimization, eliminating the need for time-consuming trial-and-error testing during routine detection while maintaining high detection accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables simultaneous detection of multiple viruses from different species with improved accuracy and reduced false-negative/false-positive outcomes, replacing costly trial-and-error testing with optimized conditions.
Implementation Method 1
coating a nucleic acid primer sequence corresponding to each of the viruses on a solid-phase carrier
Implementation Method 2
conducting an amplification reaction on the sample input into the solid-phase carrier according to the amplification reaction conditions
Data Source
AI summary
A method for virus detection is applied to detect a plurality of viruses at one time and the method comprises the following steps of: coating a nucleic acid primer sequence corresponding to each of the viruses on a solid-phase carrier, wherein the viruses originate from different species; inputting a sample into the solid-phase carrier; setting a plurality of amplification reaction conditions, wherein the amplification reaction conditions comprise an amplification reaction temperature, an amplification primer length, an amplification primer concentration and a number of amplification reaction cycles; and conducting an amplification reaction on the sample input into the solid-phase carrier according to the amplification reaction conditions, so as to detect the plurality of viruses originating from different species at one time under the amplification reaction.