Multiplexed Cytokine Assay Panel Kit
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Solution Overview
Problem
Current cytokine detection methods, such as ELISA, are limited in their ability to simultaneously measure multiple cytokines, leading to high reagent costs, excessive technician time, and the need for large sample volumes, whereas a multiplexed assay capable of detecting multiple cytokines simultaneously would reduce costs and improve efficiency.
Innovation Solution
A cytokine panel kit comprising multi-well assay plates with discrete binding domains for capture antibodies and labeled detection antibodies for various cytokines, along with calibrator proteins, optimized through CIEF, DLS, and Experion testing to ensure high specificity and efficiency, allowing for the simultaneous analysis of multiple cytokines in a single sample.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional ELISA method is used to detect cytokines, then detection specificity is maintained, but the ability to simultaneously measure multiple cytokines is limited
Solution Approach 1:
The assay system is segmented into distinct functional components: capture antibodies immobilized on plate surfaces, detection antibodies with electrochemiluminescent labels, and magnetic beads for separation. This segmentation enables multiplexing while maintaining individual antibody specificity for each cytokine target.
Solution Approach 2:
A universal detection system using electrochemiluminescent-labeled detection antibodies is designed to detect multiple different cytokines simultaneously. The same detection platform and readout method can be applied across different cytokine targets, reducing overall system complexity despite measuring multiple analytes.
2Quantity of substance
If conventional ELISA method is used, then reagent cost per analyte is controlled, but total reagent cost increases when measuring multiple cytokines
Solution Approach 1:
Multiple cytokine detection reactions are merged into a single assay well, allowing simultaneous measurement of multiple analytes using shared reagents including detection antibodies, electrochemiluminescent substrates, and buffer solutions. This combining approach reduces total reagent consumption compared to running separate ELISA assays for each cytokine.
3Loss of time
If conventional ELISA method is used, then technician time per assay is manageable, but total technician time increases for multiple cytokine measurements
Solution Approach 1:
The assay protocol is designed to perform multiple detection functions continuously in parallel within the same well. After a single incubation and washing cycle, the electrochemiluminescent signal can detect multiple cytokines simultaneously without requiring sequential processing steps for each analyte, thereby reducing technician intervention time.
4Quantity of substance
If conventional ELISA method is used, then sample volume requirements are moderate, but large sample volumes are needed to generate each result when measuring multiple cytokines
Solution Approach 1:
Multiple cytokine detections are combined in a single reaction well using a small volume of sample. The capture antibodies are pre-immobilized on the plate surface, allowing the sample to be added once and then detected for multiple cytokines simultaneously, rather than requiring separate sample additions for each cytokine measurement.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The kit enables the simultaneous quantification of multiple cytokines, reducing reagent usage and sample volume, while maintaining high assay throughput and specificity, thereby improving the efficiency and cost-effectiveness of cytokine detection.
Implementation Method 1
each well comprising ten discrete binding domains to which capture antibodies to the following human analytes are bound: IFN-gamma, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNFalpha
Implementation Method 2
The invention provides a kit for the analysis of a cytokine panel comprising: i. (a) a multi-well assay plate comprising a plurality of wells, each well comprising ten discrete binding domains to which capture antibodies
Data Source
AI summary
Described herein are kits and components thereof used for a multiplexed analysis of a set of cytokines.